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GYLTL1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GYLTL1B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GYLTL1B antibodies →
$504.00each
Catalog Number: LS-G106362-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GYLTL1B (NM_152312), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_152312

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLPRGRPRAL GAAALLLLLL LLGFLLFGGD LGCERRKPGG RAGAPGCFPG PLMPRVPPDG
61RLRRAAALDG DPGAGPGDHN RSDCGPQPPP PPKCELLHVA IVCAGHNSSR DVITLVKSML
121FYRKNPLHLH LVTDAVARNI LETLFHTWMV PAVRVSFYHA DQLKPQVSWI PNKHYSGLYG
181LMKLVLPSAL PAELARVIVL DTDVTFASDI SELWALFAHF SDTQAIGLVE NQSDWYLGNL
241WKNHRPWPAL GRGFNTGVIL LRLDRLRQAG WEQMWRLTAR RELLSLPATS LADQDIFNAV
301IKEHPGLVQR LPCVWNVQLS DHTLAERCYS EASDLKVIHW NSPKKLRVKN KHVEFFRNFY
361LTFLEYDGNL LRRELFVCPS QPPPGAEQLQ QALAQLDEED PCFEFRQQQL TVHRVHVTFL
421PHEPPPPRPH DVTLVAQLSM DRLQMLEALC RHWPGPMSLA LYLTDAEAQQ FLHFVEASPV
481LAARQDVAYH VVYREGPLYP VNQLRNVALA QALTPYVFLS DIDFLPAYSL YDYLRASIEQ
541LGLGSRRKAA LVVPAFETLR YRFSFPHSKV ELLALLDAGT LYTFRYHEWP RGHAPTDYAR
601WREAQAPYRV QWAANYEPYV VVPRDCPRYD PRFVGFGWNK VAHIVELDAQ EYELLVLPEA
661FTIHLPHAPS LDISRFRSSP TYRDCLQALK DEFHQDLSRH HGAAALKYLP ALQQPQSPAR
721G
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

721 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.