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GTSE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GTSE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112685-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GTSE1 (NM_016426), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
B99 | G-2 and S-phase expressed 1 | GTSE-1 | GTSE1 | Protein B99 homolog
Origin Species
Human
NCBI GenBank
NM_016426

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.61 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDDPKKEDIL LLADEKFDFD LSLSSSSANE DDEVFFGPFG HKERCIAASL ELNNPVPEQP
61PLPTSESPFA WSPLAGEKFV EVYKEAHLLA LHIESSSRNQ AAQAAKPEDP RSQGVERFIQ
121ESKLKINLFE KEKEMKKSPT SLKRETYYLS DSPLLGPPVG EPRLLASSPA LPSSGAQARL
181TRAPGPPHSA HALPRESCTA HAASQAATQR KPGTKLLLPR AASVRGRSIP GAAEKPKKEI
241PASPSRTKIP AEKESHRDVL PDKPAPGAVN VPAAGSHLGQ GKRAIPVPNK LGLKKTLLKA
301PGSTSNLARK SSSGPVWSGA SSACTSPAVG KAKSSEFASI PANSSRPLSN ISKSGRMGPA
361MLRPALPAGP VGASSWQAKR VDVSELAAEQ LTAPPSASPT QPQTPEGGGQ WLNSSCAWSE
421SSQLNKTRSI RRRDSCLNSK TKVMPTPTNQ FKIPKFSIGD SPDSSTPKLS RAQRPQSCTS
481VGRVTVHSTP VRRSSGPAPQ SLLSARRVSA LPTPASRRCS GLPPMTPKTM PRAVGSPLCV
541PARRRSSEPR KNSAMRTEPT RESNRKTDSR LVDVSPDRGS PPSRVPQALN FSPEESDSTF
601SKSTATEVAR EEAKPGGDAA PSEALLVDIK LEPLAVTPDA ASQPLIDLPL IDFCDTPEAH
661VAVGSESRPL IDLMTNTPDM NKNVAKPSPV VGQLIDLSSP LIQLSPEADK ENVDSPLLKF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

720 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.