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GTPBP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GTPBP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114463-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GTPBP4 (NM_012341), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CRFG | G protein-binding protein CRFG | GTPBP4 | GTP binding protein 4 | GTP-binding protein NGB | NOG1
Origin Species
Human
NCBI GenBank
NM_012341
SwissProt
Q9BZE4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAHYNFKKIT VVPSAKDFID LTLSKTQRKT PTVIHKHYQI HRIRHFYMRK VKFTQQNYHD
61RLSQILTDFP KLDDIHPFYA DLMNILYDKD HYKLALGQIN IAKNLVDNVA KDYVRLMKYG
121DSLYRCKQLK RAALGRMCTV IKRQKQSLEY LEQVRQHLSR LPTIDPNTRT LLLCGYPNVG
181KSSFINKVTR ADVDVQPYAF TTKSLFVGHM DYKYLRWQVV DTPGILDHPL EDRNTIEMQA
241ITALAHLRAA VLYVMDLSEQ CGHGLREQLE LFQNIRPLFI NKPLIVVANK CDVKRIAELS
301EDDQKIFTDL QSEGFPVIET STLTEEGVIK VKTEACDRLL AHRVETKMKG NKVNEVLNRL
361HLAIPTRRDD KERPPFIPEG VVARRKRMET EESRKKRERD LELEMGDDYI LDLQKYWDLM
421NLSEKHDKIP EIWEGHNIAD YIDPAIMKKL EELEKEEELR TAAGEYDSVS ESEDEEMLEI
481RQLAKQIREK KKLKILESKE KNTQGPRMPR TAKKVQRTVL EKEMRSLGVD MDDKDDAHYA
541VQARRSRSIT RKRKREDSAP PSSVARSGSC SRTPRDVSGL RDVKMVKKAK TMMKNAQKKM
601NRLGKKGEAD RHVFDMKPKH LLSGKRKAGK KDRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

634 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.