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GTPBP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GTPBP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111437-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GTPBP2 (NM_019096), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GTPBP2 | GTP binding protein 2 | GTP-binding protein 2
Origin Species
Human
NCBI GenBank
NM_019096
SwissProt
Q9BX10

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSRVSELFG GCCRPGGGPA VGGTLKARGA GSSSGCGGPK GKKKNGRNRG GKANNPPYLP
61PEAEDGNIEY KLKLVNPSQY RFEHLVTQMK WRLQEGRGEA VYQIGVEDNG LLVGLAEEEM
121RASLKTLHRM AEKVGADITV LREREVDYDS DMPRKITEVL VRKVPDNQQF LDLRVAVLGN
181VDSGKSTLLG VLTQGELDNG RGRARLNLFR HLHEIQSGRT SSISFEILGF NSKGEVVNYS
241DSRTAEEICE SSSKMITFID LAGHHKYLHT TFFGLTSYCP DCALLLVSAN TGIAGTTREH
301LGLALALKVP FFIVVSKIDL CAKTTVERTV RQLERVLKQP GCHKVPMLVT SEDDAVTAAQ
361QFAQSPNVTP IFTLSSVSGE SLDLLKVFLN ILPPLTNSKE QEELMQQLTE FQVDEIYTVP
421EVGTVVGGTL SSGICREGDQ LVVGPTDDGC FLELRVCSIQ RNRSACRVLR AGQAATLALG
481DFDRALLRKG MVMVSPEMNP TICSVFEAEI VLLFHATTFR RGFQVTVHVG NVRQTAVVEK
541IHAKDKLRTG EKAVVRFRFL KHPEYLKVGA KLLFREGVTK GIGHVTDVQA ITAGEAQANM
601GF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

602 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.