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GTF3C5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GTF3C5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114654-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GTF3C5 (NM_012087), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TF3C-epsilon | TFIIIC 63 kDa subunit | TFiiiC2-63 | TFIIIC63 | TFIIICepsilon | GTF3C5
Origin Species
Human
NCBI GenBank
NM_012087
SwissProt
Q9Y5Q8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MAAEAADLGL GAAVPVELRR ERRMVCVEYP GVVRDVAKML PTLGGEEGVS RIYADPTKRL
61ELYFRPKDPY CHPVCANRFS TSSLLLRIRK RTRRQKGVLG TEAHSEVTFD MEILGIISTI
121YKFQGMSDFQ YLAVHTEAGG KHTSMYDKVL MLRPEKEAFF HQELPLYIPP PIFSRLDAPV
181DYFYRPETQH REGYNNPPIS GENLIGLSRA RRPHNAIFVN FEDEEVPKQP LEAAAQTWRR
241VCTNPVDRKV EEELRKLFDI RPIWSRNAVK ANISVHPDKL KVLLPFIAYY MITGPWRSLW
301IRFGYDPRKN PDAKIYQVLD FRIRCGMKHG YAPSDLPVKA KRSTYNYSLP ITVKKTSSQL
361VTMHDLKQGL GPSGTSGARK PASSKYKLKD SVYIFREGAL PPYRQMFYQL CDLNVEELQK
421IIHRNDGAEN SCTERDGWCL PKTSDELRDT MSLMIRQTIR SKRPALFSSS AKADGGKEQL
481TYESGEDEED EEEEEEEEED FKPSDGSENE METEILDYV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

519 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.