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GTF3C3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GTF3C3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114653-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GTF3C3 (NM_012086), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GTF3C3 | TF3C-gamma | TFIIIC102 | TFIIICgamma | TFIIIC 102 kDa subunit | TFiiiC2-102
Origin Species
Human
NCBI GenBank
NM_012086
SwissProt
Q9Y5Q9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
101.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSGFSPELID YLEGKISFEE FERRREERKT REKKSLQEKG KLSAEENPDD SEVPSSSGIN
61STKSQDKDVN EGETSDGVRK SVHKVFASML GENEDDEEEE EEEEEEEEEE ETPEQPTAGD
121VFVLEMVLNR ETKKMMKEKR PRSKLPRALR GLMGEANIRF ARGEREEAIL MCMEIIRQAP
181LAYEPFSTLA MIYEDQGDME KSLQFELIAA HLNPSDTEEW VRLAEMSLEQ DNIKQAIFCY
241TKALKYEPTN VRYLWERSSL YEQMGDHKMA MDGYRRILNL LSPSDGERFM QLARDMAKSY
301YEANDVTSAI NIIDEAFSKH QGLVSMEDVN IAAELYISNK QYDKALEIIT DFSGIVLEKK
361TSEEGTSEEN KAPENVTCTI PDGVPIDITV KLMVCLVHLN ILEPLNPLLT TLVEQNPEDM
421GDLYLDVAEA FLDVGEYNSA LPLLSALVCS ERYNLAVVWL RHAECLKALG YMERAAESYG
481KVVDLAPLHL DARISLSTLQ QQLGQPEKAL EALEPMYDPD TLAQDANAAQ QELKLLLHRS
541TLLFSQGKMY GYVDTLLTML AMLLKVAMNR AQVCLISSSK SGERHLYLIK VSRDKISDSN
601DQESANCDAK AIFAVLTSVL TKDDWWNLLL KAIYSLCDLS RFQEAELLVD SSLEYYSFYD
661DRQKRKELEY FGLSAAILDK NFRKAYNYIR IMVMENVNKP QLWNIFNQVT MHSQDVRHHR
721FCLRLMLKNP ENHALCVLNG HNAFVSGSFK HALGQYVQAF RTHPDEPLYS FCIGLTFIHM
781ASQKYVLRRH ALIVQGFSFL NRYLSLRGPC QESFYNLGRG LHQLGLIHLA IHYYQKALEL
841PPLVVEGIEL DQLDLRRDIA YNLSLIYQSS GNTGMAQTLL YTYCSI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

886 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.