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GTF3C2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GTF3C2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120688-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GTF3C2 (NM_001035521), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GTF3C2 | KIAA0011 | TF3C-beta | TFIIIC-BETA | TFIIIC 110 kDa subunit | TFIIIC110
Origin Species
Human
NCBI GenBank
NM_001521
SwissProt
Q8WUA4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDTCGVGYVA LGEAGPVGNM TVVDSPGQEV LNQLDVKTSS EMTSAEASVE MSLPTPLPGF
61EDSPDQRRLP PEQESLSRLE QPDLSSEMSK VSKPRASKPG RKRGGRTRKG PKRPQQPNPP
121SAPLVPGLLD QSNPLSTPMP KKRGRKSKAE LLLLKLSKDL DRPESQSPKR PPEDFETPSG
181ERPRRRAAQV ALLYLQELAE ELSTALPAPV SCPEGPKVSS PTKPKKIRQP AACPGGEEVD
241GAPRDEDFFL QVEAEDVEES EGPSESSSEP EPVVPRSTPR GSTSGKQKPH CRGMAPNGLP
301DHIMAPVWKC LHLTKDFREQ KHSYWEFAEW IPLAWKWHLL SELEAAPYLP QEEKSPLFSV
361QREGLPEDGT LYRINRFSSI TAHPERWDVS FFTGGPLWAL DWCPVPEGAG ASQYVALFSS
421PDMNETHPLS QLHSGPGLLQ LWGLGTLQQE SCPGNRAHFV YGIACDNGCI WDLKFCPSGA
481WELPGTPRKA PLLPRLGLLA LACSDGKVLL FSLPHPEALL AQQPPDAVKP AIYKVQCVAT
541LQVGSMQATD PSECGQCLSL AWMPTRPHQH LAAGYYNGMV VFWNLPTNSP LQRIRLSDGS
601LKLYPFQCFL AHDQAVRTLQ WCKANSHFLV SAGSDRKIKF WDLRRPYEPI NSIKRFLSTE
661LAWLLPYNGV TVAQDNCYAS YGLCGIHYID AGYLGFKAYF TAPRKGTVWS LSGSDWLGTI
721AAGDISGELI AAILPDMALN PINVKRPVER RFPIYKADLI PYQDSPEGPD HSSASSGVPN
781PPKARTYTET VNHHYLLFQD TDLGSFHDLL RREPMLRMQE GEGHSQLCLD RLQLEAIHKV
841RFSPNLDSYG WLVSGGQSGL VRIHFVRGLA SPLGHRMQLE SRAHFNAMFQ PSSPTRRPGF
901SPTSHRLLPT P
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

911 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.