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GTF3C1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GTF3C1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G118542-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GTF3C1(GTF3C1) (NM_001520), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GTF3C1 | TF3C-alpha | TFIIIC 220 kDa subunit | TFIIIC box B-binding subunit | TFIIICalpha | TFIIIC220 | TFIIIC
Origin Species
Human
NCBI GenBank
NM_001520
SwissProt
Q12789

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
238.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDALESLLDE VALEGLDGLC LPALWSRLET RVPPFPLPLE PCTQEFLWRA LATHPGISFY
61EEPRERPDLQ LQDRYEEIDL ETGILESRRD PVALEDVYPI HMILENKDGI QGSCRYFKER
121KNITNDIRTK SLQPRCTMVE PFDRWGKKLI IGSLPAHAVQ ALDSPGGGSR PEAARLLLLH
181PGTARPVQVQ GELQRDLHTT AFKVDAGKLH YHRKILNKNG LITMQSHVIR LPTGAQQHSI
241LLLLNRFHVD RRSKYDILME KLSVMLSTRT NHIETLGKLR EELGLCERTF KRLYQYMLNA
301GLAKVVSLRL QEIHPECGPC KTKKGTDVMV RCLKLLKEFK RNDHDDDEDE EVISKTVPPV
361DIVFERDMLT QTYDLIERRG TKGISQAEIR VAMNVGKLEA RMLCRLLQRF KVVKGFMEDE
421GRQRTTKYIS CVFAEESDLS RQYQREKARS ELLTTVSLAS MQEESLLPEG EDTFLSESDS
481EEERSSSKRR GRGSQKDTRA SANLRPKTQP HHSTPTKGGW KVVNLHPLKK QPPSFPGAAE
541ERACQSLASR DSLLDTSSVS EPNVSFVSHC ADSNSGDIAV IEEVRMENPK ESSSSLKTGR
601HSSGQDKPHE TYRLLKRRNL IIEAVTNLRL IESLFTIQKM IMDQEKQEGV STKCCKKSIV
661RLVRNLSEEG LLRLYRTTVI QDGIKKKVDL VVHPSMDQND PLVRSAIEQV RFRISNSSTA
721NRVKTSQPPV PQGEAEEDSQ GKEGPSGSGD SQLSASSRSE SGRMKKSDNK MGITPLRNYH
781PIVVPGLGRS LGFLPKMPRL RVVHMFLWYL IYGHPASNTV EKPSFISERR TIKQESGRAG
841VRPSSSGSAW EACSEAPSKG SQDGVTWEAE VELATETVYV DDASWMRYIP PIPVHRDFGF
901GWALVSDILL CLPLSIFIQI VQVSYKVDNL EEFLNDPLKK HTLIRFLPRP IRQQLLYKRR
961YIFSVVENLQ RLCYMGVLQF GPTEKFQDKD QVFIFLKKNA VIVDTTICDP HYNLGRRRRP
1021FERRLYVLNS MQDVENYWFD LQCVCLNTPL GVVRCPRVRK NSSTDQGSDE EGSLQKEQES
1081AMDKHNLERK CAMLEYTTGS REVVDEGLIP GDGLGAAGLD SSFYGHLKRN WIWTSYIINQ
1141AKKENTAAEN GLTVRLQTFL SKRPMPLSAR GNSRLNIWGE ARVGSELCAG WEEQFEVDRE
1201PSLDRNRRVR GGKSQKRKRL KKDPGKKIKR KKKGEFPGEK SKRLRYHDEA DQSALHRMTR
1261LRVTWSMQED GLLVLCRIAS NVLNTKVKGP FVTWQVVRDI LHATFEESLD KTSHSLGRRA
1321RYIVKNPQAY LNYKVCLAEV YQDKALVGDF MNRRGDYDDP KVCANEFKEF VEKLKEKFSS
1381ALRNSNLEIP DTLQELFARY RVLAIGDEKD QTRKEDELNS VDDIHFLVLQ NLIQSTLALS
1441DSQMKSYQSF QTFRLYREYK DHVLVKAFME CQKRSLVNRR RVNHTLGPKK NRALPFVPMS
1501YQLSQTYYRI FTWRFPSTIC TESFQFLDRM RAAGKLDQPD RFSFKDQDNN EPTNDMVAFS
1561LDGPGGNCVA VLTLFSLGLI SVDVRIPEQI IVVDSSMVEN EVIKSLGKDG SLEDDEDEED
1621DLDEGVGGKR RSMEVKPAQA SHTNYLLMRG YYSPGIVSTR NLNPNDSIVV NSCQMKFQLR
1681CTPVPARLRP AAAPLEELTM GTSCLPDTFT KLINPQENTC SLEEFVLQLE LSGYSPEDLT
1741AALEILEAII ATGCFGIDKE ELRRRFSALE KAGGGRTRTF ADCIQALLEQ HQVLEVGGNT
1801ARLVAMGSAW PWLLHSVRLK DREDADIQRE DPQARPLEGS SSEDSPPEGQ APPSHSPRGT
1861KRRASWASEN GETDAEGTQM TPAKRPALQD SNLAPSLGPG AEDGAEAQAP SPPPALEDTA
1921AAGAAQEDQE GVGEFSSPGQ EQLSGQAQPP EGSEDPRGFT ESFGAANISQ AARERDCESV
1981CFIGRPWRVV DGHLNLPVCK GMMEAMLYHI MTRPGIPESS LLRHYQGVLQ PVAVLELLQG
2041LESLGCIRKR WLRKPRPVSL FSTPVVEEVE VPSSLDESPM AFYEPTLDCT LRLGRVFPHE
2101VNWNKWIHL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

2109 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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