Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GTF3 / GTF2IRD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115799-100
Catalog Number: LS-G115799
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GTF2IRD1 (NM_005685), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BEN | CREAM1 | General transcription factor 3 | GTF2IRD1 | GTF3 | MUSTRD1 | USE B1-binding protein | WBSCR12 | RBAP2 | WBS | HMusTRD1alpha1 | WBSCR11
- Origin Species
- Human
- NCBI GenBank
- NM_016328
- SwissProt
- Q9UHL9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 104.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MALLGKRCDV PTNGCGPDRW NSAFTRKDEI ITSLVSALDS MCSALSKLNA EVACVAVHDE61SAFVVGTEKG RMFLNARKEL QSDFLRFCRG PPWKDPEAEH PKKVQRGEGG GRSLPRSSLE121HGSDVYLLRK MVEEVFDVLY SEALGRASVV PLPYERLLRE PGLLAVQGLP EGLAFRRPAE181YDPKALMAIL EHSHRIRFKL KRPLEDGGRD SKALVELNGV SLIPKGSRDC GLHGQAPKVP241PQDLPPTATS SSMASFLYST ALPNHAIREL KQEAPSCPLA PSDLGLSRPM PEPKATGAQD301FSDCCGQKPT GPGGPLIQNV HASKRILFSI VHDKSEKWDA FIKETEDINT LRECVQILFN361SRYAEALGLD HMVPVPYRKI ACDPEAVEIV GIPDKIPFKR PCTYGVPKLK RILEERHSIH421FIIKRMFDER IFTGNKFTKD TTKLEPASPP EDTSAEVSRA TVLDLAGNAR SDKGSMSEDC481GPGTSGELGG LRPIKIEPED LDIIQVTVPD PSPTSEEMTD SMPGHLPSED SGYGMEMLTD541KGLSEDARPE ERPVEDSHGD VIRPLRKQVE LLFNTRYAKA IGISEPVKVP YSKFLMHPEE601LFVVGLPEGI SLRRPNCFGI AKLRKILEAS NSIQFVIKRP ELLTEGVKEP IMDSQERDSG661DPLVDESLKR QGFQENYDAR LSRIDIANTL REQVQDLFNK KYGEALGIKY PVQVPYKRIK721SNPGSVIIEG LPPGIPFRKP CTFGSQNLER ILAVADKIKF TVTRPFQGLI PKPDEDDANR781LGEKVILREQ VKELFNEKYG EALGLNRPVL VPYKLIRDSP DAVEVTGLPD DIPFRNPNTY841DIHRLEKILK AREHVRMVII NQLQPFAEIC NDAKVPAKDS SIPKRKRKRV SEGNSVSSSS901SSSSSSSSNP DSVASANQIS LVQWPMYMVD YAGLNVQLPG PLNYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
944 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.