Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GTF2A1L / ALF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115016-100
Catalog Number: LS-G115016
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ALF(GTF2A1L) (NM_006872), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ALF | GTF2A1-like factor | GTF2A1LF | GTF2A1L | TFIIA-alpha/beta-like factor
- Origin Species
- Human
- NCBI GenBank
- NM_006872
- SwissProt
- Q9UNN4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MACLNPVPKL YRSVIEDVIE GVRNLFAEEG IEEQVLKDLK QLWETKVLQS KATEDFFRNS61IQSPLFTLQL PHSLHQTLQS STASLVIPAG RTLPSFTTAE LGTSNSSANF TFPGYPIHVP121AGVTLQTVSG HLYKVNVPIM VTETSGRAGI LQHPIQQVFQ QLGQPSVIQT SVPQLNPWSL181QATTEKSQRI ETVLQQPAIL PSGPVDRKHL ENATSDILVS PGNEHKIVPE ALLCHQESSH241YISLPGVVFS PQVSQTNSNV ESVLSGSASM AQNLHDESLS TSPHGALHQH VTDIQLHILK301NRMYGCDSVK QPRNIEEPSN IPVSEKDSNS QVDLSIRVTD DDIGEIIQVD GSGDTSSNEE361IGSTRDADEN EFLGNIDGGD LKVPEEEADS ISNEDSATNS SDNEDPQVNI VEEDPLNSGD421DVSEQDVPDL FDTDNVIVCQ YDKIHRSKNK WKFYLKDGVM CFGGRDYVFA KAIGDAEWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
478 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.