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GSTCD Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GSTCD Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G120717-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GSTCD (NM_001031720), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GSTCD
Origin Species
Human
NCBI GenBank
NM_024751
SwissProt
Q8NEC7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKAIKKSLTE EEYLYLDFSH QTEGCIFPLH TSVTLFLLSY CDCKIFKICL VVTKEVSRDS
61SLLRDDLIQD VEIQIISRQE LPPIVQNCCL PAVVERSDNF CRAGLAVVLR HIIQKSYEAD
121PLKKELLELL GFKKTCLKAC AEVSQWTRLC ELTIPLAIEN FLRESSDQPP TIPVEILQLE
181KKLSEPVRVH NDDKLRRQKL KQQKADGVGP PLTKGKAKSK VHTQETSEGL DSSSKSLELK
241VAFSKLTVQE EPATTNREPS HIRKAKASDL PPLEHVFAEG LYFTLADIVL LPCIHHFLVI
301ISRKFSEKLV EFPLLASWYQ RIQEVPGVKT ASKCGIQFLH LPKLLTTSTE QHPNLCEVPG
361VEEQSDPLFI GGPRPTMAKL MEKGIEVMFS PHPCPTWTLD WNVLPAAVSP KEGKMSSDRA
421LRKQQQLNNL VYVVTNQAKP GDRIVDFCSG GGHVGIVLAH MLPSCQVTLI ENKELSLIRA
481KKRSDELGLS NIWFIQANME YFTGMFNIGV ALHACGVATD MVIEHCIKTR ASFVTCPCCY
541GFIQNTSKFN FPKSEQFKKT LSYKEHMILC RFADQTAVQL PPQRRLIGKQ CMCLVDLDRA
601RAAEECGYSV QVISMEPESC SPKNNMIVGV PI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

632 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.