Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GSDMB / Gasdermin-Like Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128108-100
Catalog Number: LS-G128108
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Gasdermin like(GSDMB) (NM_001165959), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Gasdermin-like protein | GSDMB | Gasdermin-B | Gasdermin-like | GSDML | Gasdermin B | PP4052 | PRO2521
- Origin Species
- Human
- NCBI GenBank
- NM_018530
- SwissProt
- Q8TAX9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFSVFEEITR IVVKEMDAGG DMIAVRSLVD ADRFRCFHLV GEKRTFFGCR HYTTGLTLMD61ILDTDGDKWL DELDSGLQGQ KAEFQILDNV DSTGELIVRL PKEITISGSF QGFHHQKIKI121SENRISQQYL ATLENRKLKR ELPFSFRSIN TRENLYLVTE TLETVKEETL KSDRQYKFWS181QISQGHLSYK HKGQREVTIP PNRVLSYRVK QLVFPNKETM NIHFRGKTKS FPEGKSLGSE241DSRNMKEKLE DMESVLKDLT EEKRKDVLNS LAKCLGKEDI RQDLEQRVSE VLISGELHME301DPDKPLLSSL FNAAGVLVEA RAKAILDFLD ALLELSEEQQ FVAEALEKGT LPLLKDQVKS361VMEQNWDELA SSPPDMDYDP EARILCALYV VVSILLELAE GPTSVSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
407 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.