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GRIP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GRIP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GRIP2 antibodies →
$725.00each
Catalog Number: LS-G120211-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRIP2 (NM_001080423), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GRIP-2 | GRIP2 | KIAA1719
Origin Species
Human
NCBI GenBank
NM_001080423

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
122.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRLSSLTEEA VDGGNGGEWG EDLLEQFTCI PSRVVEAKTA PEWPLCPGEG QGLHGEPLPD
61HCQGTGGQID PSLGRGGRAK QRGSRGSWWA AGETIANMLC GLSRETPGEA DDGPYSKGGK
121DAGGADVSLA CRRQSIPEEF RGITVVELIK KEGSTLGLTI SGGTDKDGKP RVSNLRPGGL
181AARSDLLNIG DYIRSVNGIH LTRLRHDEII TLLKNVGERV VLEVEYELPP PAPENNPRII
241SKTVDVSLYK EGNSFGFVLR GGAHEDGHKS RPLVLTYVRP GGPADREGSL KVGDRLLSVD
301GIPLHGASHA TALATLRQCS HEALFQVEYD VATPDTVANA SGPLMVEIVK TPGSALGISL
361TTTSLRNKSV ITIDRIKPAS VVDRSGALHP GDHILSIDGT SMEHCSLLEA TKLLASISEK
421VRLEILPVPQ SQRPLRPQRQ VVKVQRSEQL HRWDPCVPSC HSPRPGHCRM PTWATPAGQD
481QSRSLSSTPF SSPTLNHAFS CNNPSTLPRG SQPMSPRTTM GRRRQRRREH KSSLSLASST
541VGPGGQIVHT ETTEVVLCGD PLSGFGLQLQ GGIFATETLS SPPLVCFIEP DSPAERCGLL
601QVGDRVLSIN GIATEDGTME EANQLLRDAA LAHKVVLEVE FDVAESVIPS SGTFHVKLPK
661KRSVELGITI SSASRKRGEP LIISDIKKGS VAHRTGTLEP GDKLLAIDNI RLDNCPMEDA
721VQILRQCEDL VKLKIRKDED NSDELETTGA VSYTVELKRY GGPLGITISG TEEPFDPIVI
781SGLTKRGLAE RTGAIHVGDR ILAINNVSLK GRPLSEAIHL LQVAGETVTL KIKKQLDRPL
841LPRKSGSLSE TSDADEDPAD ALKGGLPAAR FSPAVPSVDS AVESWDSSAT EGGFGGPGSY
901TPQAAARGTT PQERRPGWLR GSPPPTEPRR TSYTPTPADE SFPEEEEEDD WEPPTSPAPG
961PAREEGFWRM FGEALEDLES CGQSELLREL EASIMTGTVQ RVALEGRPGH RPWQRGREVR
1021ASPAEMEELL LPTPLEMHKV TLHKDPMRHD FGFSVSDGLL EKGVYVHTVR PDGPAHRGGL
1081QPFDRVLQVN HVRTRDFDCC LAVPLLAEAG DVLELIISRK PHTAHSSRAP RSPGPSSPRM
1141L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1141 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.