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GRIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GRIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GRIP1 antibodies →
$725.00each
Catalog Number: LS-G110745-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRIP1 (NM_021150), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GRIP | GRIP-1 | GRIP1
Origin Species
Human
NCBI GenBank
AJ133439
SwissProt
Q9Y3R0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
116.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIAVSFKCRC QILRRLTKDE SPYTKSASQT KPPDGALAVR RQSIPEEFKG STVVELMKKE
61GTTLGLTVSG GIDKDGKPRV SNLRQGGIAA RSDQLDVGDY IKAVNGINLA KFRHDEIISL
121LKNVGERVVL EVEYELPPVS VQGSSVIFRT VEVTLHKEGN TFGFVIRGGA HDDRNKSRPV
181VITCVRPGGP ADREGTIKPG DRLLSVDGIR LLGTTHAEAM SILKQCGQEA ALLIEYDVSV
241MDSVATASGP LLVEVAKTPG ASLGVALTTS MCCNKQVIVI DKIKSASIAD RCGALHVGDH
301ILSIDGTSME YCTLAEATQF LANTTDQVKL EILPHHQTRL ALKGPDHAAL VSSSFSPTSM
361SAYSLSSLNM GTLPRSLYST SPRGTMMRRR LKKKDFKSSL SLASSTVGLA GQVVHTETTE
421VVLTADPVTG FGIQLQGSVF ATETLSSPPL ISYIEADSPA ERCGVLQIGD RVMAINGIPT
481EDSTFEEASQ LLRDSSITSK VTLEIEFDVA ESVIPSSGTF HVKLPKKHNV ELGITISSPS
541SRKPGDPLVI SDIKKGSVAH RTGTLELGDK LLAIDNIRLD NCSMEDAVQI LQQCEDLVKL
601KIRKDEDNSD EQESSGAIIY TVELKRYGGP LGITISGTEE PFDPIIISSL TKGGLAERTG
661AIHIGDRILA INSSSLKGKP LSEAIHLLQM AGETVTLKIK KQTDAQSASS PKKFPISSHL
721SDLGDVEEDS SPAQKPGKLS DMYPSTVPSV DSAVDSWDGS AIDTSYGTEG TSFQASGYNF
781NTYDWRSPKQ RGSLSPVTKP RSQTYPDVGL SYEDWDRSTA SGFAGAADSA ETEQEENFWS
841QALEDLETCG QSGILRELEE KADRRVSLRN MTLLATIMSG STMSLNHEAP TPRSQLGRQA
901SFQERSSSRP HYSQTTRSNT LPSDVGRKSV TLRKMKQEIK EIMSPTPVEL HKVTLYKDSD
961MEDFGFSVAD GLLEKGVYVK NIRPAGPGDL GGLKPYDRLL QVNHVRTRDF DCCLVVPLIA
1021ESGNKLDLVI SRNPLASQKS IDQQSLPGDW SEQNSAFFQQ PSHGGNLETR EPTNTL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1076 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.