Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GRIN3A / NR3A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107493-100
Catalog Number: LS-G107493
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRIN3A(GRIN3A) (NM_133445), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GluN3A | GRIN3A | NMDAR-L | KIAA1973 | NMDAR3A | NR3A
- Origin Species
- Human
- NCBI GenBank
- NM_133445
- SwissProt
- Q8TCU5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 125.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRRLSLWWLL SRVCLLLPPP CALVLAGVPS SSSHPQPCQI LKRIGHAVRV GAVHLQPWTT61APRAASRAPD DSRAGAQRDE PEPGTRRSPA PSPGARWLGS TLHGRGPPGS RKPGEGARAE121ALWPRDALLF AVDNLNRVEG LLPYNLSLEV VMAIEAGLGD LPLLPFSSPS SPWSSDPFSF181LQSVCHTVVV QGVSALLAFP QSQGEMMELD LVSLVLHIPV ISIVRHEFPR ESQNPLHLQL241SLENSLSSDA DVTVSILTMN NWYNFSLLLC QEDWNITDFL LLTQNNSKFH LGSIINITAN301LPSTQDLLSF LQIQLESIKN STPTVVMFGC DMESIRRIFE ITTQFGVMPP ELRWVLGDSQ361NVEELRTEGL PLGLIAHGKT TQSVFEHYVQ DAMELVARAV ATATMIQPEL ALIPSTMNCM421EVETTNLTSG QYLSRFLANT TFRGLSGSIR VKGSTIVSSE NNFFIWNLQH DPMGKPMWTR481LGSWQGGKIV MDYGIWPEQA QRHKTHFQHP SKLHLRVVTL IEHPFVFTRE VDDEGLCPAG541QLCLDPMTND SSTLDSLFSS LHSSNDTVPI KFKKCCYGYC IDLLEKIAED MNFDFDLYIV601GDGKYGAWKN GHWTGLVGDL LRGTAHMAVT SFSINTARSQ VIDFTSPFFS TSLGILVRTR661DTAAPIGAFM WPLHWTMWLG IFVALHITAV FLTLYEWKSP FGLTPKGRNR SKVFSFSSAL721NICYALLFGR TVAIKPPKCW TGRFLMNLWA IFCMFCLSTY TANLAAVMVG EKIYEELSGI781HDPKLHHPSQ GFRFGTVRES SAEDYVRQSF PEMHEYMRRY NVPATPDGVE YLKNDPEKLD841AFIMDKALLD YEVSIDADCK LLTVGKPFAI EGYGIGLPPN SPLTANISEL ISQYKSHGFM901DMLHDKWYRV VPCGKRSFAV TETLQMGIKH FSGLFVLLCI GFGLSILTTI GEHIVYRLLL961PRIKNKSKLQ YWLHTSQRLH RAINTSFIEE KQQHFKTKRV EKRSNVGPRQ LTVWNTSNLS1021HDNRRKYIFS DEEGQNQLGI QIHQDIPLPP RRRELPALRT TNGKADSLNV SRNSVMQELS1081ELEKQIQVIR QELQLAVSRK TELEEYQRTS RTCESTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1115 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.