Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GRIK4 / KA1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113836-100
Catalog Number: LS-G113836
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KA1(GRIK4) (NM_014619), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GRIK | KA1 | GluK4 | Glutamate receptor KA-1 | Glutamate receptor KA1 | GRIK4 | EAA1
- Origin Species
- Human
- NCBI GenBank
- NM_014619
- SwissProt
- Q16099
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 107.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPRVSAPLVL LPAWLVMVAC SPHSLRIAAI LDDPMECSRG ERLSITLAKN RINRAPERLG61KAKVEVDIFE LLRDSEYETA ETMCQILPKG VVAVLGPSSS PASSSIISNI CGEKEVPHFK121VAPEEFVKFQ FQRFTTLNLH PSNTDISVAV AGILNFFNCT TACLICAKAE CLLNLEKLLR181QFLISKDTLS VRMLDDTRDP TPLLKEIRDD KTATIIIHAN ASMSHTILLK AAELGMVSAY241YTYIFTNLEF SLQRMDSLVD DRVNILGFSI FNQSHAFFQE FAQSLNQSWQ ENCDHVPFTG301PALSSALLFD AVYAVVTAVQ ELNRSQEIGV KPLSCGSAQI WQHGTSLMNY LRMVELEGLT361GHIEFNSKGQ RSNYALKILQ FTRNGFRQIG QWHVAEGLSM DSHLYASNIS DTLFNTTLVV421TTILENPYLM LKGNHQEMEG NDRYEGFCVD MLKELAEILR FNYKIRLVGD GVYGVPEANG481TWTGMVGELI ARKADLAVAG LTITAEREKV IDFSKPFMTL GISILYRVHM GRKPGYFSFL541DPFSPGVWLF MLLAYLAVSC VLFLVARLTP YEWYSPHPCA QGRCNLLVNQ YSLGNSLWFP601VGGFMQQGST IAPRALSTRC VSGVWWAFTL IIISSYTANL AAFLTVQRMD VPIESVDDLA661DQTAIEYGTI HGGSSMTFFQ NSRYQTYQRM WNYMYSKQPS VFVKSTEEGI ARVLNSNYAF721LLESTMNEYY RQRNCNLTQI GGLLDTKGYG IGMPVGSVFR DEFDLAILQL QENNRLEILK781RKWWEGGKCP KEEDHRAKGL GMENIGGIFV VLICGLIVAI FMAMLEFLWT LRHSEATEVS841VCQEMVTELR SIILCQDSIH PRRRRAAVPP PRRPIPEERR PRGTATLSNG KLCGAGEPDQ901LAQRLAQEAA LVARGCTHIR VCPECRRFQG LRARPSPARS EESLEWEKTT NSSEPETRTRPLEQKLISEEDLAANDILDYKDDDDKV
956 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.