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GRID2IP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GRID2IP Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GRID2IP antibodies →
$725.00each
Catalog Number: LS-G126534-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRID2IP (NM_001145118), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GRID2IP | Delphilin
Origin Species
Human
SwissProt
A4D2P6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
132.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATTATPATN QGWPEDFGFR LGGSGPCFVL EVAKGSSAHA GGLRPGDQIL EVEGLAVGGL
61SRERLVRLAR RCPRVPPSLG VLPAPDGGPG PGSGPAAPTT VLRAPRCGRG LALGRELLRL
121AGRKRPDAVH RERRRKAQEF SRKVDEILGD QPTAKEQVFA ALKQFAAEQR VDDLVWTLTL
181ALPREACGPL LDNLRIFIPK KHRARFDEVV SQGLLGKLCR ARRAQGAQRL RRSRSEERPE
241RLLVSTRASA PPRRPDEPPP RRASLLVGGL AGPGGARRTV RVYKGNKSFG FTLRGHGPVW
301IESVLPGSPA DNAALKSGDR ILFLNGLDMR NCSHDKVVSM LQGSGAMPTL VVEEGLVPFA
361SDSDSLDSPN PSSALTSLQW VAEILPSSIR VQGRTFSQQL EHLLTPPERY GVCRALESFF
421QHRNIDTLIV DVYPVLDTPA KQVLWQFIYQ LLTYEEQELC QEKIACFLGY TAMTAEPEPE
481LDLESEPTPE PQPRSSLRAS SMCRRSLRSQ GLEAGLSCGP SECPEMPLPL IPGERQAGDG
541TSLPETPNPK MMSAVYAELE SRLNSSFKGK MGTVSKSRAS PPGPSPAVTT GPRTLSGVSW
601PSERLLPSPC YHPLCSGGLA SPSSSESHPY ASLDSSRAPS PQPGPGPICP DSPPSPDPTR
661PPSRRKLFTF SHPVRSRDTD RFLDVLSEQL GPRVTIVDDF LTPENDYEEM SFHDDQGSFV
721TNERSSASDC ISSSEEGSSL TYSSISDHIP PPPLSPPPPP PLPFHDAKPS SRSSDGSRGP
781AQALAKPLTQ LSHPVPPPPP PPLPPPVPCA PPMLSRGLGH RRSETSHMSV KRLRWEQVEN
841SEGTIWGQLG EDSDYDKLSD MVKYLDLELH FGTQKPAKPV PGPEPFRKKE VVEILSHKKA
901YNTSILLAHL KLSPAELRQV LMSMEPRRLE PAHLAQLLLF APDADEEQRY QAFREAPGRL
961SEPDQFVLQM LSVPEYKTRL RSLHFQATLQ EKTEEIRGSL ECLRQASLEL KNSRKLAKIL
1021EFVLAMGNYL NDGQPKTNKT TGFKINFLTE LNSTKTVDGK STFLHILAKS LSQHFPELLG
1081FAQDLPTVPL AAKVNQRALT SDLADLHGTI SEIQDACQSI SPSSEDKFAM VMSSFLETAQ
1141PALRALDGLQ REAMEELGKA LAFFGEDSKA TTSEAFFGIF AEFMSKFERA LSDLQAGEGL
1201RSSGMVSPLA W
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1211 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.