Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GRIA2 / GLUR2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119829-100
Catalog Number: LS-G119829
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Ionotropic Glutamate receptor 2(GRIA2) (NM_001083620), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AMPA 2 | GluR-K2 | Glutamate receptor 2 | GRIA2 | HBGR2 | GluA2 | GluR-2 | GluR-B | GLUR2 | GLURB
- Origin Species
- Human
- NCBI GenBank
- NM_000826
- SwissProt
- P42262
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 93.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVQFSTSEFR LTPHIDNLEV ANSFAVTNAF CSQFSRGVYA IFGFYDKKSV NTITSFCGTL61HVSFITPSFP TDGTHPFVIQ MRPDLKGALL SLIEYYQWDK FAYLYDSDRG LSTLQAVLDS121AAEKKWQVTA INVGNINNDK KDEMYRSLFQ DLELKKERRV ILDCERDKVN DIVDQVITIG181KHVKGYHYII ANLGFTDGDL LKIQFGGANV SGFQIVDYDD SLVSKFIERW STLEEKEYPG241AHTTTIKYTS ALTYDAVQVM TEAFRNLRKQ RIEISRRGNA GDCLANPAVP WGQGVEIERA301LKQVQVEGLS GNIKFDQNGK RINYTINIME LKTNGPRKIG YWSEVDKMVV TLTELPSGND361TSGLENKTVV VTTILESPYV MMKKNHEMLE GNERYEGYCV DLAAEIAKHC GFKYKLTIVG421DGKYGARDAD TKIWNGMVGE LVYGKADIAI APLTITLVRE EVIDFSKPFM SLGISIMIKK481PQKSKPGVFS FLDPLAYEIW MCIVFAYIGV SVVLFLVSRF SPYEWHTEEF EDGRETQSSE541STNEFGIFNS LWFSLGAFMR QGCDISPRSL SGRIVGGVWW FFTLIIISSY TANLAAFLTV601ERMVSPIESA EDLSKQTEIA YGTLDSGSTK EFFRRSKIAV FDKMWTYMRS AEPSVFVRTT661AEGVARVRKS KGKYAYLLES TMNEYIEQRK PCDTMKVGGN LDSKGYGIAT PKGSSLRTPV721NLAVLKLSEQ GVLDKLKNKW WYDKGECGAK DSGSKEKTSA LSLSNVAGVF YILVGGLGLA781MLVALIEFCY KSRAEAKRMK VAKNAQNINP SSSQNSQNFA TYKEGYNVYG IESVKITRTRPLEQKLISEEDLAANDILDYKDDDDKV
836 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.