Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GRHL1 / TFCP2L2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129068-100
Catalog Number: LS-G129068
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Grainyhead like protein 1 homolog(GRHL1) (NM_198182), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Grainyhead-like 1 (Drosophila) | MGR | NH32 | LBP-32 | TFCP2L2 | Mammalian grainyhead | Transcription factor LBP-32 | GRHL1 | LBP protein 32 | LBP32
- Origin Species
- Human
- NCBI GenBank
- NM_014552
- SwissProt
- Q9NZI5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTQEYDNKRP VLVLQNEALY PQRRSYTSED EAWKSFLENP LTAATKAMMS INGDEDSAAA61LGLLYDYYKV PRERRSSTAK PEVEHPEPDH SKRNSIPIVT EQPLISAGEN RVQVLKNVPF121NIVLPHGNQL GIDKRGHLTA PDTTVTVSIA TMPTHSIKTE TQPHGFAVGI PPAVYHPEPT181ERVVVFDRNL NTDQFSSGAQ APNAQRRTPD STFSETFKEG VQEVFFPSDL SLRMPGMNSE241DYVFDSVSGN NFEYTLEASK SLRQKPGDST MTYLNKGQFY PITLKEVSSS EGIHHPISKV301RSVIMVVFAE DKSREDQLRH WKYWHSRQHT AKQRCIDIAD YKESFNTISN VEEIAYNAIS361FTWDINDEAK VFISVNCLST DFSSQKGVKG LPLNIQIDTY SYNNRSNKPV HRAYCQIKVF421CDKGAERKIR DEERKQSKRK VSDVKVPLLP SHKRMDITVF KPFIDLDTQP VLFIPDVHFA481NLQRGTHVLP IASEELEGEG SVLKRGPYGT EDDFAVPPST KLARIEEPKR VLLYVRKESE541EVFDALMLKT PSLKGLMEAI SDKYDVPHDK IGKIFKKCKK GILVNMDDNI VKHYSNEDTF601QLQIEEAGGS YKLTLTEITRTRPLEQKLISEEDLAANDILDYKDDDDKV
618 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.