Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GREB1L / KIAA1772 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G126142-100
Catalog Number: LS-G126142
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GREB1L (NM_001142966), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1772 | GREB1-like protein | C18orf6 | GREB1L
- Origin Species
- Human
- NCBI GenBank
- NM_024935
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 214.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGNSYAGQLK SARFEEALHN SIEASLRCSS VVPRPIFSQL YLDPDQHPFS SADVKPKVED61LDKDLVNRYT QNGSLDFSNN LTVNEMEDDE DDEEMSDSNS PPIPYSQKPA PEGSCTTDGF121CQAGKDLRLV SLCMEQIDIP AGFLLVGAKS PNLPEHILVC AVDKRFLPDD HGKNALLGFS181GNCIGCGERG FRYFTEFSNH INLKLTTQPK KQKHLKYYLV RSSQGVLSKG PLICWKECRS241RQSSASCHSI KPSSSVSSTV TPENGTTNGY KSGFTQTDAA NGNSSHGGKG SASSSTPAHT301GNYSLSPRPS YASGDQATMF ISGPPKKRHR GWYPGSPLPQ PGLVVPVPTV RPLSRTEPLL361SAPVPQTPLT GILQPRPIPA GETVIVPENL LSNSGVRPVI LIGYGTLPYF YGNVGDIVVS421PLLVNCYKIP QLENKDLEKL GLTGSQFLSV ENMILLTIQY LVRLGPDQVP LREEFEQIML481KAMQEFTLRE RALQIGAQCV PVSPGQLPWL ARLIASVSQD LVHVVVTQNS LAEGISETLR541TLSEMRHYQR LPDYVVVICA SKIRGNEFCV VVLGQHQSRA LAESMLTTSE FLKEISYELI601TGKVSFLASH FKTTSLGDDL DKLLEKMQQR RGDSVVTPFD GDLNECVSPQ EAAAMIPTQN661LDLDNETFHI YQPQLTVARK LLSQVCAIAD SGSQSLDLGH FSKVDFIIIV PRSEVLVQQT721LQRIRQSGVL VDLGLEENGT AHQRAEKYVV RLDNEIQTKF EVFMRRVKQN PYTLFVLVHD781NSHVELTSVI SGSLSHSEPS HGLADRVINC REVLEAFNLL VLQVSSFPYT LQTQQSRISS841SNEVHWIQLD TGEDVGCEEK LYFGLSEYSK SLQWGITSPL LRCDETFEKM VNTLLERYPR901LHSMVVRCYL LIQQYSEALM ALTTMASLRD HSTPETLSIM DDLISSPGKN KSGRGHMLII961RVPSVQLAML AKERLQEVRD KLGLQYRFEI ILGNPATELS VATHFVARLK SWRGNEPEEW1021IPRTYQDLDG LPCIVILTGK DPLGETFPRS LKYCDLRLID SSYLTRTALE QEVGLACCYV1081SKEVIRGPTV ALDLSGKEQE RAAVSENDSD ELLIDLERPQ SNSSAVTGTS GSIMENGVSS1141SSTADKSQKQ SLTPSFQSPA TSLGLDEGVS ASSAGAGAGE TLKQECDSLG PQMASSTTSK1201PSSSSSGPRT LPWPGQPIRG CRGPQAALPP VVILSKAAYS LLGSQKSGKL PSSSSLLPHA1261DVAWVSSLRP LLNKDMSSEE QSLYYRQWTL ARQHHADYSN QLDPASGTRN FHPRRLLLTG1321PPQVGKTGSY LQFLRILFRM LIRLLEVDVY DEEEINTDHN ESSEVSQSEG EPWPDIESFS1381KMPFDVSVHD PKYSLMSLVY TEKLAGVKQE VIKESKVEEP RKRETVSIML TKYAAYNTFH1441HCEQCRQYMD FTSASQMSDS TLHAFTFSSS MLGEEVQLYF IIPKSKESHF VFSKQGKHLE1501SMRLPLVSDK NLNAVKSPIF TPSSGRHEHG LLNLFHAMEG ISHLHLLVVK EYEMPLYRKY1561WPNHIMLVLP GMFNNAGVGA ARFLIKELSY HNLELERNRL EELGIKRQCV WPFIVMMDDS1621CVLWNIHSVQ EPSSQPMEVG VSSKNVSLKT VLQHIEATPK IVHYAILGIQ KWSSKLTSQS1681LKAPFSRCHV HDFILLNTDL TQNVQYDFNR YFCEDADFNL RTNSSGLLIC RFNNFSLMKK1741HVQVGGQRDF IIKPKIMVSE SLAPILPLQY ICAPDSEHTL LAAPAQFLLE KFLQHASYKL1801FPKAIHNFRS PVLAIDCYLN IGPEVAICYI SSRPHSSNVN CEGVFFSGLL LYLCDSFVGA1861DLKKFKFLKG ATLCVICQDR SSLRQTIVRL ELEDEWQFRL RDEFQTANSS DDKPLYFLTG1921RHVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1923 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.