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GREB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GREB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GREB1 antibodies →
$725.00each
Catalog Number: LS-G113788-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GREB1 (NM_014668), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0575 | Protein GREB1 | GREB1
Origin Species
Human
NCBI GenBank
NM_014668
SwissProt
Q4ZG55

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
216.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGNSYAGQLK TTRFEEVLHN SIEASLRSNN LVPRPIFSQL YLEAEQQLAA LEGGSRVDNE
61EEEEEGEGGL ETNGPPTPFQ LHPLPEGCCT TDGFCQAGKD LRLVSISNEP MDVPAGFLLV
121GVKSPSLPDH LLVCAVDKRF LPDDNGHNAL LGFSGNCVGC GKKGFCYFTE FSNHINLKLT
181TQPKKQKHLK YYLVRNAQGT LTKGPLICWK GSEFRSRQIP ASTCSSSLFP ALESTAAFPS
241EPVPGTNPSI LMGAQQAGPA SDHPSLNAAM GPAVFNGKDS PKCQQLAKNN LLALPRPSAL
301GILSNSGPPK KRHKGWSPES PSAPDGGCPQ GGGNRAKYES AGMSCVPQVG LVGPASVTFP
361VVASGEPVSV PDNLLKICKA KPVIFKGHGN FPYLCGNLND VVVSPLLYTC YQNSQSVSRA
421YEQYGASAIQ PISEEMQLLL TVYYLVQLAA DQVPLMEDLE QIFLRSWRES HLTEIRQYQQ
481APPQPFPPAP SAAAPVTSAQ LPWLASLAAS SCNDSVHVIE CAYSLAEGLS EMFRLLVEGK
541LAKTNYVVII CACRSAAIDS CIAVTGKYQA RILSESLLTP AEYQKEVNYE LVTGKVDSLG
601AFFSTLCPEG DIDILLDKFH QENQGHISSS LAASSVTKAA SLDVSGTPVC TSYNLEPHSI
661RPFQLAVAQK LLSHVCSIAD SSTQNLDLGS FEKVDFLICI PPSEVTYQQT LLXVWHSGVL
721LELGLKKEHM TKQRVEQYVL KLDTEAQTKF KAFLQNSFQN PHTLFVLIHD HAHWDLVSST
781VHNLYSQSDP SVGLVDRLLN CREVKEAPNI VTLHVTSFPY ALQTQHTLIS PYNEIHWPAS
841CSNGVDLYHE NKKYFGLSEF IESTLSGHSL PLLRYDSSFE AMVTALGKRF PRLHSAVIRT
901FVLVQHYAAA LMAVSGLPQM KNYTSVETLE ITQNLLNSPK QCPCGHGLMV LLRVPCSPLA
961VVAYERLAHV RARLALEEHF EIILGSPSSG VTVGKHFVKQ LRMWQKIEDV EWRPQTYLEL
1021EGLPCILIFS GMDPHGESLP RSLRYCDLRL INSSCLVRTA LEQELGLAAY FVSNEVPLEK
1081GARNEALESD AEKLSSTDNE DEELGTEGST SEKRSPMKRE RSRSHDSASS SLSSKASGSA
1141LGGESSAQPT ALPQGEHARS PQPRGPAEEG RAPGEKQRPR ASQGPPSAIS RHSPGPTPQP
1201DCSLRTGQRS VQVSVTSSCS QLSSSSGSSS SSVAPAAGTW VLQASQCSLT KACRQPPIVF
1261LPKLVYDMVV STDSSGLPKA ASLLPSPSVM WASSFRPLLS KTMTSTEQSL YYRQWTVPRP
1321SHMDYGNRAE GRVDGFHPRR LLLSGPPQIG KTGAYLQFLS VLSRMLVRLT EVDVYDEEEI
1381NINLREESDW HYLQLSDPWP DLELFKKLPF DYIIHDPKYE DASLICSHYQ GIKSEDRGMS
1441RKPEDLYVRR QTARMRLSKY AAYNTYHHCE QCHQYMGFHP RYQLYESTLH AFAFSYSMLG
1501EEIQLHFIIP KSKEHHFVFS QPGGQLESMR LPLVTDKSHE YIKSPTFTPT TGRHEHGLFN
1561LYHAMDGASH LHVLVVKEYE MAIYKKYWPN HIMLVLPSIF NSAGVGAAHF LIKELSYHNL
1621ELERNRQEEL GIKPQDIWPF IVISDDSCVM WNVVDVNSAG ERSREFSWSE RNVSLKHIMQ
1681HIEAAPDIMH YALLGLRKWS SKTRASEVQE PFSRCHVHNF IILNVDLTQN VQYNQNRFLC
1741DDVDFNLRVH SAGLLLCRFN RFSVMKKQIV VGGHRSFHIT SKVSDNSAAV VPAQYICAPD
1801SKHTFLAAPA QLLLEKFLQH HSHLFFPLSL KNHDHPVLSV DCYLNLGSQI SVCYVSSRPH
1861SLNISCSDLL FSGLLLYLCD SFVGASFLKK FHFLKGATLC VICQDRSSLR QTVVRLELED
1921EWQFRLRDEF QTANAREDRP LFFLTGRHI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1949 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.