LSBio
GRASP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GRASP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GRASP antibodies →
$504.00each
Catalog Number: LS-G104417-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human General Receptor for phosphoinositides 1(GRASP) (NM_181711), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GRASP | TAMALIN
Origin Species
Human
NCBI GenBank
NM_019518
SwissProt
Q9JJA9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
42.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTLRRLRKLQ QKEEAAATPD PAARTPDSEV APAAPVPTPG PPAAAATPGP PADELYAALE
61DYHPAELYRA LAVSGGTLPR RKGSGFRWKN LSQSPEQQRK VLTLEKEDNQ TFGFEIQTYG
121LHHREEQRVE MVTFVCRVHE SSPAQLAGLT PGDTIASVNG LNVEGIRHRE IVDIIKASGN
181VLRLETLYGT SIRKAELEAR LQYLKQTLYE KWGEYRSLMV QEQRLVHGLV VKDPSIYDTL
241ESVRSCLYGA GLLPGSLPFG PLLAVPGRPR GGARRARGDA DDAVYHTCFF GDSEPPALPP
301PPPPARAFGP GPAETPAVGP GPGPRAALSR SASVRCAGPG GGGGGGAPGA LWTEAREQAL
361CGPGLRKTKY RSFRRRLLKF IPGLNRSLEE EESQL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

395 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.