Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GRAP2 / GRID Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100395-100
Catalog Number: LS-G100395
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRAP2 (NM_004810), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Adapter protein GRID | GRAP-2 | GRB2L | Grf40 adapter protein | GRB2-related adapter protein 2 | GRBLG | Grf-40 | Grf40 | GRB2-related adaptor protein 2 | GRID | MONA | Protein GADS | SH3-SH2-SH3 adaptor molecule | SH3-SH2-SH3 adapter Mona | GADS | GRAP2 | GRB-2-like protein | GrbX | GRPL | p38
- Origin Species
- Human
- NCBI GenBank
- NM_004810
- SwissProt
- O75791
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAVAKFDFT ASGEDELSFH TGDVLKILSN QEEWFKAELG SQEGYVPKNF IDIQFPKWFH61EGLSRHQAEN LLMGKEVGFF IIRASQSSPG DFSISVRHED DVQHFKVMRD NKGNYFLWTE121KFPSLNKLVD YYRTNSISRQ KQIFLRDRTR EDQGHRGNSL DRRSQGGPHL SGAVGEEIRP181SMNRKLSDHP PTLPLQQHQH QPQPPQYAPA PQQLQQPPQQ RYLQHHHFHQ ERRGGSLDIN241DGHCGTGLGS EMNAALMHRR HTDPVQLQAA GRVRWARALY DFEALEDDEL GFHSGEVVEV301LDSSNPSWWT GRLHNKLGLF PANYVAPMTRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
330 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.