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GRAMD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GRAMD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GRAMD4 antibodies →
$725.00each
Catalog Number: LS-G113452-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRAMD4 (NM_015124), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_015124

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MLRRLDKIRF RGHKRDDFLD LAESPNASDT ECSDEIPLKV PRTSPRDSEE LRDPAGPGTL
61IMATGVQDFN RTEFDRLNEI KGHLEIALLE KHFLQEELRK LREETNAEML RQELDRERQR
121RMELEQKVQE VLKARTEEQM AQQPPKGQAQ ASNGAERRSQ GLSSRLQKWF YERFGEYVED
181FRFQPEENTV ETEEPLSARR LTENMRRLKR GAKPVTNFVK NLSALSDWYS VYTSAIAFTV
241YMNAVWHGWA IPLFLFLAIL RLSLNYLIAR GWRIQWSIVP EVSEPVEPPK EDLTVSEKFQ
301LVLDVAQKAQ NLFGKMADIL EKIKNLFMWV QPEITQKLYV ALWAAFLASC FFPYRLVGLA
361VGLYAGIKFF LIDFIFKRCP RLRAKYDTPY IIWRSLPTDP QLKERSSAAV SRRLQTTSSR
421SYVPSAPAGL GKEEDAGRFH STKKGNFHEI FNLTENERPL AVCENGWRCC LINRDRKMPT
481DYIRNGVLYV TENYLCFESS KSGSSKRNKV IKLVDITDIQ KYKVLSVLPG SGMGIAVSTP
541STQKPLVFGA MVHRDEAFET ILSQYIKITS AAASGGDS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

578 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.