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GRAMD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GRAMD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GRAMD3 antibodies →
$504.00each
Catalog Number: LS-G128142-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRAMD3 (NM_001146319), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GRAM domain containing 3 | NS3TP2 | GRAMD3
Origin Species
Human
NCBI GenBank
NM_023927
SwissProt
Q96HH9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
49.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTRRQQVLQA KRSIQQTFQA QLMPWKSMFH GREVKPVGPD LELSEAENGV EEKKKACRSP
61TAQSPTPSVE ADSPDQKKII SLWSKSSFDG ASLASDKNDC KTESKNDPKT ERKKSSSSSQ
121YKANMHFHKL FLSVPTEEPL KQSFTCALQK EILYQGKLFV SENWICFHSK VFGKDTKISI
181PAFSVTLIKK TKTALLVPNA LIIATVTDRY IFVSLLSRDS TYKLLKSVCG HLENTSVGNS
241PNPSSAENSF RADRPSSLPL DFNDEFSDLD GVVQQRRQDM EGYSSSGSQT PESENSRDFH
301ATESQTVLNV SKGEAKPTRA DAHVNRVPEG KAKSLPVQGL SETVGILHKV KSQKCPMLHH
361ILIFYAIVVC ALIISTFYMR YRINTLEEQL GLLTSIVDTH NTEQAAPSGL RSQVQFNVEV
421LCQELTANIV KLEKIQNNLQ KLLENGD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

447 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.