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GRAMD1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GRAMD1B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GRAMD1B antibodies →
$725.00each
Catalog Number: LS-G111028-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRAMD1B (NM_020716), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GRAM domain containing 1B | GRAMD1B | KIAA1201
Origin Species
Human
NCBI GenBank
AB033027
SwissProt
Q3KR37

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKGFKLSCTA SNSNRSTPAC SPILRKRSRS PTPQNQDGDT MVEKGSDHSS DKSPSTPEQG
61VQRSCSSQSG RSGGKNSKKS QSWYNVLSPT YKQRNEDFRK LFKQLPDTER LIVDYSCALQ
121RDILLQGRLY LSENWICFYS NIFRWETLLT VRLKDICSMT KEKTARLIPN AIQVCTDSEK
181HFFTSFGARD RTYMMMFRLW QNALLEKPLC PKELWHFVHQ CYGNELGLTS DDEDYVPPDD
241DFNTMGYCEE IPVEENEVND SSSKSSIETK PDASPQLPKK SITNSTLTST GSSEAPVSFD
301GLPLEEEALE GDGSLEKELA IDNIMGEKIE MIAPVNSPSL DFNDNEDIPT ELSDSSDTHD
361EGEVQAFYED LSGRQYVNEV FNFSVDKLYD LLFTNSPFQR DFMEQRRFSD IIFHPWKKEE
421NGNQSRVILY TITLTNPLAP KTATVRETQT MYKASQESEC YVIDAEVLTH DVPYHDYFYT
481INRYTLTRVA RNKSRLRVST ELRYRKQPWG LVKTFIEKNF WSGLEDYFRH LESELAKTES
541TYLAEMHRQS PKEKASKTTT VRRRKRPHAH LRVPHLEEVM SPVTTPTDED VGHRIKHVAG
601STQTRHIPED TPNGFHLQSV SKLLLVISCV ICFSLVLLVI LNMMLFYKLW MLEYTTQTLT
661AWQGLRLQER LPQSQTEWAQ LLESQQKYHD TELQKWREII KSSVMLLDQM KDSLINLQNG
721IRSRDYTSES EEKRNRYH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

738 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.