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GRAMD1A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GRAMD1A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GRAMD1A antibodies →
$725.00each
Catalog Number: LS-G125688-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GRAMD1A (NM_001136199), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GRAM domain containing 1A | GRAMD1A | KIAA1533
Origin Species
Human
NCBI GenBank
NM_001136199
SwissProt
Q96CP6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MFDTTPHSGR STPSSSPSLR KRLQLLPPSR PPPEPEPGTM VEKGSDSSSE KGGVPGTPST
61QSLGSRNFIR NSKMLSPTYK QRNEDFRKLF SKLPEAERLI VDYSCALQRE ILLQGRLYLS
121ENWICFYSNI FRWETTISIQ LKEVTCLKKE KTAKLIPNAI QICTESEKHF FTSFGARDRC
181FLLIFRLWQN ALLEKTLSPR ELWHLVHQCY GSELGLTSED EDYVSPLQLN GLGTPKEVGD
241VIALSDITSS GAADRSQEPS PVGSRRGHVT PNLSRASSDA DHGAEEDKEE QVDSQPDASS
301SQTVTPVAEP PSTEPTQPDG PTTLGPLDLL PSEELLTDTS NSSSSTGEEA DLAALLPDLS
361GRLLINSVFH VGAERLQQML FSDSPFLQGF LQQCKFTDVT LSPWSGDSKC HQRRVLTYTI
421PISNPLGPKS ASVVETQTLF RRGPQAGGCV VDSEVLTQGI PYQDYFYTAH RYCILGLARN
481KARLRVSSEI RYRKQPWSLV KSLIEKNSWS GIEDYFHHLE RELAKAEKLS LEEGGKDARG
541LLSGLRRRKR PLSWRAHGDG PQHPDPDPCA RAGIHTSGSL SSRFSEPSVD QGPGAGIPSA
601LVLISIVLII LIALNVLLFY RLWSLERTAH TFESWHSLAL AKGKFPQTAT EWAEILALQK
661QFHSVEVHKW RQILRASVEL LDEMKFSLEK LHQGITVSDP PFDTQPRPDD SFS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

713 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.