Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GRAB / RAB3IL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114286-100
Catalog Number: LS-G114286
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAB3IL1 (NM_013401), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Rabin3-like 1 | GRAB | RAB3IL1
- Origin Species
- Human
- NCBI GenBank
- NM_013401
- SwissProt
- Q8TBN0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MWSGPPQPDQ GLPPPLAAVP VPWKSTDPCQ GHRESPGALV ETSAGEEAQG QEGPAAAQLD61VLRLRSSSME IREKGSEFLK EELHRAQKEL KLKDEECERL SKVREQLEQE LEELTASLFE121EAHKMVREAN MKQAASEKQL KEARGKIDML QAEVTALKTL VITSTPASPN RELHPQLLSP181TKAGPRKGHS RHKSTSSTLC PAVCPAAGHT LTPDREGKEV DTILFAEFQA WRESPTLDKT241CPFLERVYRE DVGPCLDFTM QELSVLVRAA VEDNTLTIEP VASQTLPTVK VAEVDCSSTN301TCALSGLTRT CRHRIRLGDS KSHYYISPSS RARITAVCNF FTYIRYIQQG LVRQDAEPMF361WEIMRLRKEM SLAKLGFFPQ EATRTRPLEQKLISEEDLAANDILDYKDDDDKV
382 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.