Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPSM1 / AGS3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G126771-100
Catalog Number: LS-G126771
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPSM1 (NM_001145638), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AGS3 homolog | DKFZP727I051 | AGS3 | GPSM1
- Origin Species
- Human
- NCBI GenBank
- NM_015597
- SwissProt
- Q86YR5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGPAPPVAD ELPGPAARRL YSRMEASCLE LALEGERLCK AGDFKTGVAF FEAAVQVGTE61DLKTLSAIYS QLGNAYFYLK EHGRALEYHK HDLLLARTIG DRMGEAKASG NLGNTLKVLG121RFDEAAVCCQ RHLSIAQEQG DKVGEARALY NIGNVYHAKG KQLSWNAANA TQDPGHLPPD181VRETLCKASE FYERNLSLVK ELGDRAAQGR AYGNLGNTHY LLGNFTEATT FHKERLAIAK241EFGDKAAERR AYSNLGNAHV FLGRFDVAAE YYKKTLQLSR QLRDQAVEAQ ACYSLGNTYT301LLQDYERAAE YHLRHLLIAQ ELADRVGEGR ACWSLGNAYV SMGRPAQALT FAKKHLQISQ361EIGDRHGELT ARMNVAQLQL VLGRLTSPAA SEKPDLAGYE AQGARPKRTQ RLSAETWDLL421RLPLEREQNG DSHHSGDWRG PSRDSLPLPV RSRKYQEGPD AERRPREGSH SPLDSADVRV481HVPRTSIPRA PSSDEECFFD LLTKFQSSRM DDQRCPLDDG QAGAAEATAA PTLEDRIAQP541SMTASPQTEE FFDLIASSQS RRLDDQRASV GSLPGLRITH SNAGHLRGHG EPQEPGDDFF601NMLIKYQSSR IDDQRCPPPD VLPRGPTMPD EDFFSLIQRV QAKRMDEQRV DLAGGPEQGA661GGPPEPQQQC QPGASTRTRPLEQKLISEEDLAANDILDYKDDDDKV
675 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.