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GPRIN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPRIN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103770-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPRIN3 (NM_198281), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GRIN3 | GPRIN3 | GPRIN family member 3 | KIAA2027
Origin Species
Human
SwissProt
Q6ZVF9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGTVPDPLRS AKTSLIAASG KEDDLGEPQA ASPRHRPALL CKNANGFSGA PAEPDLSPRA
61AAEALMQVCE HETTQPDMSS PGVFNEVQKA PATFNSPGNP QLPGSSQPAA SAPSSAAGRD
121LIHTPLTMPA NQHTCQSIPG DQPNAITSSM PEDSLMRSQR TSNREQPEKP SCPVGGVLSS
181SKDQVSCEFP SPETIQGTVQ TPVTAARVVS HSSSPVGGPE GERQGAICDS EMRSCKPLTR
241ESGCSENKQP SVTASGPQGT TSVTPQPTPL TSEPSACPPG PEKVPLPAQR QMSRFKEAST
301MTNQAESEIK EVPSRAWQDA EVQAVASVES RSVSTSPSIL TAFLKESRAP EHFEQEQLRV
361ICHSSGSHTL ELSDSTLAPQ EPSQCPGIMP QVHIQAAAAE STAFQRENKL ASLPGGVLKT
421SSINLVSSNA QHTCKEDGRL AGMTPAREES TAKKLAGTNS SSLKATAIDQ ISISACSQAE
481TSYGLGKFET RPSEFAEKTT NGHKTDPDCK LSDSCGSISK ADHSGSLDPT NKGDAREKKP
541ASPQVVKEKE STGTDTSDAK TLLLNPKSQE SGGTESAANP TPSPIRKNQE STLEENRQTK
601TATSLSLPSD PMGDSSPGSG KKTPSRSVKA SPRRPSRVSE FLKEQKLNVT AAAAQVGLTP
661GDKKKQLGAD SKLQLKQSKR VRDVVWDEQG MTWEVYGASL DAESLGIAIQ NHLQRQIREH
721EKLIKTQNSQ TRRSISSDTS SNKKLRGRQH SVFQSMLQNF RRPNCCVRPA PSSVLD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

776 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.