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GPRC6A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPRC6A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106430-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPRC6A (NM_148963), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA86F4.3 | GPRC6A | HGPCR33 | HGPRC6A | Predicted with SOSUI analysis
Origin Species
Human
NCBI GenBank
NM_148963
SwissProt
Q5T6X5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
104.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAFLIILITC FVIILATSQP CQTPDDFVAA TSPGHIIIGG LFAIHEKMLS SEDSPRRPQI
61QECVGFEISV FLQTLAMIHS IEMINNSTLL SGVKLGYEIY DTCTEVTVAM AATLRFLSKF
121NCSRETVEFK CDYSSYMPRV KAVIGSGYSE ITMAVSRMLN LQLMPQVGYE STAEILSDKI
181RFPSFLRTVP SDFHQIKAMA HLIQKSGWNW IGIITTDDDY GRLALNTFII QAEANNVCIA
241FKEVLPAFLS DNTIEVRINR TLKKIILEAQ VNVIVVFLRQ FHVFDLFNKA IEMNINKMWI
301ASDNWSTATK ITTIPNVKKI GKVVGFAFRR GNISSFHSFL QNLHLLPSDS HKLLHEYAMH
361LSACAYVKDT DLSQCIFNHS QRTLAYKANK AIERNFVMRN DFLWDYAEPG LIHSIQLAVF
421ALGYAIRDLC QARDCQNPNA FQPWELLGVL KNVTFTDGWN SFHFDAHGDL NTGYDVVLWK
481EINGHMTVTK MAEYDLQNDV FIIPDQETKN EFRNLKQIQS KCSKECSPGQ MKKTTRSQHI
541CCYECQNCPE NHYTNQTDMP HCLLCNNKTH WAPVRSTMCF EKEVEYLNWN DSLAILLLIL
601SLLGIIFVLV VGIIFTRNLN TPVVKSSGGL RVCYVILLCH FLNFASTSFF IGEPQDFTCK
661TRQTMFGVSF TLCISCILTK SLKILLAFSF DPKLQKFLKC LYRPILIIFT CTGIQVVICT
721LWLIFAAPTV EVNVSLPRVI ILECEEGSIL AFGTMLGYIA ILAFICFIFA FKGKYENYNE
781AKFITFGMLI YFIAWITFIP IYATTFGKYV PAVEIIVILI SNYGILYCTF IPKCYVIICK
841QEINTKSAFL KMIYSYSSHS VSSIALSPAS LDSMSGNVTM TNPSSSGKSA TWQKSKDLQA
901QAFAHICREN ATSVSKTLPR KRMSSI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

926 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.