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GPRC5C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPRC5C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101782-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPRC5C (NM_022036), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GPRC5C | RAIG-3 | RAIG3
Origin Species
Human
NCBI GenBank
NM_018653
SwissProt
Q9NQ84

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRGRGSQQQQ PTRRQGQKLP SPSPAGKYES AQPGGTQPEP GLGARMAIHK ALVMCLGLPL
61FLFPGAWAQG HVPPGCSQGL NPLYYNLCDR SGAWGIVLEA VAGAGIVTTF VLTIILVASL
121PFVQDTKKRS LLGTQVFFLL GTLGLFCLVF ACVVKPDFST CASRRFLFGV LFAICFSCLA
181AHVFALNFLA RKNHGPRGWV IFTVALLLTL VEVIINTEWL IITLVRGSGE GGPQGNSSAG
241WAVASPCAIA NMDFVMALIY VMLLLLGAFL GAWPALCGRY KRWRKHGVFV LLTTATSVAI
301WVVWIVMYTY GNKQHNSPTW DDPTLAIALA ANAWAFVLFY VIPEVSQVTK SSPEQSYQGD
361MYPTRGVGYE TILKEQKGQS MFVENKAFSM DEPVAAKRPV SPYSGYNGQL LTSVYQPTEM
421ALMHKVPSEG AYDIILPRAT ANSQVMGSAN STLRAEDMYS AQSHQAATPP KDGKNSQVFR
481NPYVWD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

486 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.