LSBio
GPR89A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPR89A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GPR89A antibodies →
$504.00each
Catalog Number: LS-G119035-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR89A (NM_001097613), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G protein-coupled receptor 89A | Golgi pH regulator A | GPR89A | GPHRA | GPR89 | Protein GPR89 | Protein GPR89A | SH120 | UNQ192
Origin Species
Human
NCBI GenBank
NM_001097612
SwissProt
P0CG08

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
49.99 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRQLFKDYEI RQYVVQVIFS VTFAFSCTMF ELIIFEILGV LNSSSRYFHW KMNLCVILLI
61LVFMVPFYIG YFIVSNIRLL HKQRLLFSCL LWLTFMYFFW KLGDPFPILS PKHGILSIEQ
121LISRVGVIGV TLMALLSGFG AVNCPYTYMS YFLRNVTDTD ILALERRLLQ TMDMIISKKK
181RMAMARRTMF QKGEVHNKPS GFWGMIKSVT TSASGSENLT LIQQEVDALE ELSRQLFLET
241ADLYATKERI EYSKTFKGKY FNFLGYFFSI YCVWKIFMAT INIVFDRVGK TDPVTRGIEI
301TVNYLGIQFD VKFWSQHISF ILVGIIIVTS IRGLLITLTK FFYAISSSKS SNVIVLLLAQ
361IMGMYFVSSV LLIRMSMPLE YRTIITEVLG ELQFNFYHRW FDVIFLVSAL SSILFLYLAH
421KQAPEKQMAP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

430 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.