Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPR80 / GPR99 / OXGR1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102203-100
Catalog Number: LS-G102203
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OXGR1 (NM_080818), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 2-oxoglutarate receptor 1 | Alpha-ketoglutarate receptor 1 | G protein-coupled receptor 80 | GPR80 | G-protein coupled receptor 99 | GPR99 | OXGR1 | p2Y purinoceptor 15 | p2Y-like nucleotide receptor | p2RY15 | Oxoglutarate receptor 1 | p2Y-like GPCR | p2Y15 | G protein-coupled receptor 99 | G-protein coupled receptor 80
- Origin Species
- Human
- NCBI GenBank
- NM_080818
- SwissProt
- Q96P68
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNEPLDYLAN ASDFPDYAAA FGNCTDENIP LKMHYLPVIY GIIFLVGFPG NAVVISTYIF61KMRPWKSSTI IMLNLACTDL LYLTSLPFLI HYYASGENWI FGDFMCKFIR FSFHFNLYSS121ILFLTCFSIF RYCVIIHPMS CFSIHKTRCA VVACAVVWII SLVAVIPMTF LITSTNRTNR181SACLDLTSSD ELNTIKWYNL ILTATTFCLP LVIVTLCYTT IIHTLTHGLQ TDSCLKQKAR241RLTILLLLAF YVCFLPFHIL RVIRIESRLL SISCSIENQI HEAYIVSRPL AALNTFGNLL301LYVVVSDNFQ QAVCSTVRCK VSGNLEQAKK ISYSNNPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
337 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.