LSBio
GPR75 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPR75 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GPR75 antibodies →
$504.00each
Catalog Number: LS-G115081-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR75 (NM_006794), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G protein-coupled receptor 75 | GPR-chr2 | GPR75 | WI31133 | GPRchr2 | WI-31133
Origin Species
Human
NCBI GenBank
NM_006794
SwissProt
O95800

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNSTGHLQDA PNATSLHVPH SQEGNSTSLQ EGLQDLIHTA TLVTCTFLLA VIFCLGSYGN
61FIVFLSFFDP AFRKFRTNFD FMILNLSFCD LFICGVTAPM FTFVLFFSSA SSIPDAFCFT
121FHLTSSGFII MSLKTVAVIA LHRLRMVLGK QPNRTASFPC TVLLTLLLWA TSFTPATLAT
181LKTSKSHLCL PMSSLIAGKG KAILSLYVVD FTFCVAVVSV SYIMIAQTLR KNAQVRKCPP
241VITVDASRPQ PFMGVPVQGG GDPIQCAMPA LYRNQNYDKL QHVQTRGYTK SPNQLVTPAA
301SRLQLVSAIN LSTAKDSKAV VTCVIIVLSV LVCCLPLGIS LVQVVLSSNG SFILYQFELF
361GFTLIFFKSG LNPFIYSRNS AGLRRKVLWC LQYIGLGFFC CKQKTRLRAM GKGNLEVNRN
421KSSHHETNSA YMLSPKPQKK FVDQACGPSH SKESMVSPKI SAGHQHCGQS SSTPINTRIE
481PYYSIYNSSP SQEESSPCNL QPVNSFGFAN SYIAMHYHTT NDLVQEYDST SAKQIPVPSV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

540 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.