Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPR44 / CRTH2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100390-100
Catalog Number: LS-G100390
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR44(PTGDR2) (NM_004778), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD294 | CRTH2 | CD294 antigen | DP2 | DL1R | G protein-coupled receptor 44 | G-protein coupled receptor 44 | HCRTH2 | PTGDR2 | DP2 prostaglandin receptor | GPR44 | Prostaglandin D2 receptor 2
- Origin Species
- Human
- NCBI GenBank
- NM_004778
- SwissProt
- Q9Y5Y4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSANATLKPL CPILEQMSRL QSHSNTSIRY IDHAAVLLHG LASLLGLVEN GVILFVVGCR61MRQTVVTTWV LHLALSDLLA SASLPFFTYF LAVGHSWELG TTFCKLHSSI FFLNMFASGF121LLSAISLDRC LQVVRPVWAQ NHRTVAAAHK VCLVLWALAV LNTVPYFVFR DTISRLDGRI181MCYYNVLLLN PGPDRDATCN SRQAALAVSK FLLAFLVPLA IIASSHAAVS LRLQHRGRRR241PGRFVRLVAA VVAAFALCWG PYHVFSLLEA RAHANPGLRP LVWRGLPFVT SLAFFNSVAN301PVLYVLTCPD MLRKLRRSLR TVLESVLVDD SELGGAGSSR RRRTSSTARS ASPLALCSRP361EEPRGPARLL GWLLGSCAAS PQTGPLNRAL SSTSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
395 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.