Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPR182 / ADMR Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101005-100
Catalog Number: LS-G101005
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR182 (NM_007264), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 7TMR | Adrenomedullin L1 receptor | AM-R | AMR | ADMR | Adrenomedullin receptor | G protein-coupled receptor 182 | GPR182 | HrhAMR | G-protein coupled receptor 182 | Gamrh | HAMR | RAMR | G10D
- Origin Species
- Human
- NCBI GenBank
- NM_007264
- SwissProt
- O15218
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSVKPSWGPG PSEGVTAVPT SDLGEIHNWT ELLDLFNHTL SECHVELSQS TKRVVLFALY61LAMFVVGLVE NLLVICVNWR GSGRAGLMNL YILNMAIADL GIVLSLPVWM LEVTLDYTWL121WGSFSCRFTH YFYFVNMYSS IFFLVCLSVD RYVTLTSASP SWQRYQHRVR RAMCAGIWVL181SAIIPLPEVV HIQLVEGPEP MCLFMAPFET YSTWALAVAL STTILGFLLP FPLITVFNVL241TACRLRQPGQ PKSRRHCLLL CAYVAVFVMC WLPYHVTLLL LTLHGTHISL HCHLVHLLYF301FYDVIDCFSM LHCVINPILY NFLSPHFRGR LLNAVVHYLP KDQTKAGTCA SSSSCSTQHS361IIITKGDSQP AAAAPHPEPS LSFQAHHLLP NTSPISPTQP LTPSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
404 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.