Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPR173 / SREB3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111535-100
Catalog Number: LS-G111535
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SREB3(GPR173) (NM_018969), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein coupled receptor 173 | G-protein coupled receptor 173 | GPR173 | G protein-coupled receptor 173 | SREB3
- Origin Species
- Human
- NCBI GenBank
- NM_018969
- SwissProt
- Q9NS66
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MANTTGEPEE VSGALSPPSA SAYVKLVLLG LIMCVSLAGN AILSLLVLKE RALHKAPYYF61LLDLCLADGI RSAVCFPFVL ASVRHGSSWT FSALSCKIVA FMAVLFCFHA AFMLFCISVT121RYMAIAHHRF YAKRMTLWTC AAVICMAWTL SVAMAFPPVF DVGTYKFIRE EDQCIFEHRY181FKANDTLGFM LMLAVLMAAT HAVYGKLLLF EYRHRKMKPV QMVPAISQNW TFHGPGATGQ241AAANWIAGFG RGPMPPTLLG IRQNGHAASR RLLGMDEVKG EKQLGRMFYA ITLLFLLLWS301PYIVACYWRV FVKACAVPHR YLATAVWMSF AQAAVNPIVC FLLNKDIKKC LRTHAPCWGT361GGAPAPREPY CVMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
373 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.