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GPR161 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPR161 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102406-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR161 (NM_153832), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G-protein coupled receptor 161 | G protein-coupled receptor 161 | G-protein coupled receptor RE2 | GPR161 | RE2
Origin Species
Human
NCBI GenBank
NM_007369
SwissProt
Q8N6U8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLNSSLSCR KELSNLTEEE GGEGGVIITQ FIAIIVITIF VCLGNLVIVV TLYKKSYLLT
61LSNKFVFSLT LSNFLLSVLV LPFVVTSSIR REWIFGVVWC NFSALLYLLI SSASMLTLGV
121IAIDRYYAVL YPMVYPMKIT GNRAVMALVY IWLHSLIGCL PPLFGWSSVE FDEFKWMCVA
181AWHREPGYTA FWQIWCALFP FLVMLVCYGF IFRVARVKAR KVHCGTVVIV EEDAQRTGRK
241NSSTSTSSSG SRRNAFQGVV YSANQCKALI TILVVLGAFM VTWGPYMVVI ASEALWGKSS
301VSPSLETWAT WLSFASAVCH PLIYGLWNKT VRKELLGMCF GDRYYREPFV QRQRTSRLFS
361ISNRITDLGL SPHLTALMAG GQPLGHSSST GDTGFSCSQD SGTDMMLLED YTSDDNPPSH
421CTCPPKRRSS VTFEDEVEQI KEAAKNSILH VKAEVHKSLD SYAASLAKAI EAEAKINLFG
481EEALPGVLVT ARTVPGGGFG GRRGSRTLVS QRLQLQSIEE GDVLAAEQR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

529 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.