LSBio
GPR156 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPR156 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GPR156 antibodies →
$725.00each
Catalog Number: LS-G105919-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR156 (NM_153002), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G protein-coupled receptor 156 | GABABL | GPR156 | PGR28
Origin Species
Human
NCBI GenBank
NM_153002
SwissProt
Q8NFN8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
88.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPEINCSEL CDSFPGQELD RRPLHDLCKT TITSSHHSSK TISSLSPVLL GIVWTFLSCG
61LLLILFFLAF TIHCRKNRIV KMSSPNLNIV TLLGSCLTYS SAYLFGIQDV LVGSSMETLI
121QTRLSMLCIG TSLVFGPILG KSWRLYKVFT QRVPDKRVII KDLQLLGLVA ALLMADVILL
181MTWVLTDPIQ CLQILSVSMT VTGKDVSCTS TSTHFCASRY SDVWIALIWG CKGLLLLYGA
241YLAGLTGHVS SPPVNQSLTI MVGVNLLVLA AGLLFVVTRY LHSWPNLVFG LTSGGIFVCT
301TTINCFIFIP QLKQWKAFEE ENQTIRRMAK YFSTPNKSFH TQYGEEENCH PRGEKSSMER
361LLTEKNAVIE SLQEQVNNAK EKIVRLMSAE CTYDLPEGAA PPASSPNKDV QAVASVHTLA
421AAQGPSGHLS DFQNDPGMAA RDSQCTSGPS SYAQSLEGPG KDSSFSPGKE EKISDSKDFS
481DHLDSGCSQK PWTEQSLGPE RGDQVPMNPS QSLLPDRGGS DPQRQRHLEN SEEPPERRSR
541VSSVIREKLQ EVLQDLGLGP EASLSTAPSC HQQTWKNSAA FSPQKMPLSK ELGFSPYMVR
601RRRAAQRARS HFPGSAPSSV GHRANRTVPG AHSRLHVQNG DSPSLAPQTT DSRVRRPSSR
661KPSLPSDPQD RPGTLEGSKQ SQTEPEGARG SKAAFLRQPS GSGRAPSPAA PCLSKASPDL
721PEQWQLWPPV PSGCASLSSQ HSYFDTESSS SDEFFCRCHR PYCEICFQSS SDSSDSGTSD
781TDPEPTGGLA SWEKLWARSK PIVNFKDDLK PTLV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

814 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.