LSBio
GPR152 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPR152 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GPR152 antibodies →
$725.00each
Catalog Number: LS-G102905-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR152 (NM_206997), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G protein-coupled receptor 152 | GPR152 | Heat-stable fragment rp8-9 | PGR5
Origin Species
Human
NCBI GenBank
NM_206997
SwissProt
Q8TDT2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDTTMEADLG ATGHRPRTEL DDEDSYPQGG WDTVFLVALL LLGLPANGLM AWLAGSQARH
61GAGTRLALLL LSLALSDFLF LAAAAFQILE IRHGGHWPLG TAACRFYYFL WGVSYSSGLF
121LLAALSLDRC LLALCPHWYP GHRPVRLPLW VCAGVWVLAT LFSVPWLVFP EAAVWWYDLV
181ICLDFWDSEE LSLRMLEVLG GFLPFLLLLV CHVLTQATAC RTCHRQQQPA ACRGFARVAR
241TILSAYVVLR LPYQLAQLLY LAFLWDVYSG YLLWEALVYS DYLILLNSCL SPFLCLMASA
301DLRTLLRSVL SSFAAALCEE RPGSFTPTEP QTQLDSEGPT LPEPMAEAQS QMDPVAQPQV
361NPTLQPRSDP TAQPQLNPTA QPQSDPTAQP QLNLMAQPQS DSVAQPQADT NVQTPAPAAS
421SVPSPCDEAS PTPSSHPTPG ALEDPATPPA SEGESPSSTP PEAAPGAGPT
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

470 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.