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GPR119 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPR119 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102489-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR119 (NM_178471), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G-protein coupled receptor 2 | GPCR2 | HGPCR2 | G protein-coupled receptor 119 | G-protein coupled receptor 119 | GPR119
Origin Species
Human
NCBI GenBank
NM_178471
SwissProt
Q8TDV5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
36.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESSFSFGVI LAVLASLIIA TNTLVAVAVL LLIHKNDGVS LCFTLNLAVA DTLIGVAISG
61LLTDQLSSPS RPTQKTLCSL RMAFVTSSAA ASVLTVMLIT FDRYLAIKQP FRYLKIMSGF
121VAGACIAGLW LVSYLIGFLP LGIPMFQQTA YKGQCSFFAV FHPHFVLTLS CVGFFPAMLL
181FVFFYCDMLK IASMHSQQIR KMEHAGAMAG GYRSPRTPSD FKALRTVSVL IGSFALSWTP
241FLITGIVQVA CQECHLYLVL ERYLWLLGVG NSLLNPLIYA YWQKEVRLQL YHMALGVKKV
301LTSFLLFLSA RNCGPERPRE SSCHIVTISS SEFDG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

335 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.