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GPR108 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPR108 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119327-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR108 (NM_001080452), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
G protein-coupled receptor 108 | GPR108 | LUSTR2 | Protein GPR108
Origin Species
Human
NCBI GenBank
AX073578
SwissProt
Q9NPR9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVSERRGLG RGSPAEWGQR LLLVLLLGGC SGRIHQLALT GEKRADIQLN SFGFYTNGSL
61EVELSVLRLG LREAEEKSLL VGFSLSRVRS GRVRSYSTRD FQDCPLQKNS SSFLVLFLIN
121TKDLQVQVRK YGEQKTLFIF PGLLPEAPSK PGLPKPQATV PRKVDGGGTS AASKPKSTPA
181VIQGPSGKDK DLVLGLSHLN NSYNFSFHVV IGSQAEEGQY SLNFHNCNNS VPGKEHPFDI
241TVMIREKNPD GFLSAAEMPL FKLYMVMSAC FLAAGIFWVS ILCRNTYSVF KIHWLMAALA
301FTKSISLLFH SINYYFINSQ GHPIEGLAVM YYIAHLLKGA LLFITIALIG SGWAFIKYVL
361SDKEKKVFGI VIPMQVLANV AYIIIESREE GASDYVLWKE ILFLVDLICC GAILFPVVWS
421IRHLQDASGT DGKVAVNLAK LKLFRHYYVM VICYVYFTRI IAILLQVAVP FQWQWLYQLL
481VEGSTLAFFV LTGYKFQPTG NNPYLQLPQE DEEDVQMEQV MTDSGFREGL SKVNKTASGR
541ELL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

543 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.