Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPR107 / LUSTR1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125763-100
Catalog Number: LS-G125763
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR107 (NM_001136558), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA138E2.2 | G protein-coupled receptor 107 | KIAA1624 | LUSTR1 | GPR107 | Protein GPR107 | GCDRP | RP11-88G17
- Origin Species
- Human
- NCBI GenBank
- NM_020960
- SwissProt
- Q5VW38
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAALAPVGSP ASRGPRLAAG LRLLPMLGLL QLLAEPGLGR VHHLALKDDV RHKVHLNTFG61FFKDGYMVVN VSSLSLNEPE DKDVTIGFSL DRTKNDGFSS YLDEDVNYCI LKKQSVSVTL121LILDISRSEV RVKSPPEAGT QLPKIIFSRD EKVLGQSQEP NVNPASAGNQ TQKTQDGGKS181KRSTVDSKAM GEKSFSVHNN GGAVSFQFFF NISTDDQEGL YSLYFHKCLG KELPSDKFTF241SLDIEITEKN PDSYLSAGEI PLPKLYISMA FFFFLSGTIW IHILRKRRND VFKIHWLMAA301LPFTKSLSLV FHAIDYHYIS SQGFPIEGWA VVYYITHLLK GALLFITIAL IGTGWAFIKH361ILSDKDKKIF MIVIPLQVLA NVAYIIIEST EEGTTEYGLW KDSLFLVDLL CCGAILFPVV421WSIRHLQEAS ATDGKGDSMG PLQQRANLRA GSRIAAINLA KLKLFRHYYV LIVCYIYFTR481IIAFLLKLAV PFQWKWLYQL LDETATLVFF VLTGYKFRPA SDNPYLQLSQ EEEDLEMESV541VTTSGVMESM KKVKKVTNGS VEPQGEWEGA VTRTRPLEQKLISEEDLAANDILDYKDDDDKV
571 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.