Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPN1 / XAB1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126494-100
Catalog Number: LS-G126494
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human XAB1(GPN1) (NM_001145047), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ATP(GTP)-binding protein | ATPBD1A | GPN-loop GTPase 1 | GPN1 | MBD2-interacting protein | RPAP4 | XPA binding protein 1 | GTPase | MBDIN | NTPBP | XAB1 | XPA-binding protein 1
- Origin Species
- Human
- NCBI GenBank
- NM_007266
- SwissProt
- Q9HCN4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTGHTRSSLP RCTGIVLLIK LRFSERLTGH LHAQGTPPYV INLDPAVHEV PFPANIDIRD61TVKYKEVMKQ YGLGPNGGIV TSLNLFATRF DQVMKFIEKA QNMSKYVLID TPGQIEVFTW121SASGTIITEA LASSFPTVVI YVMDTSRSTN PVTFMSNMLY ACSILYKTKL PFIVVMNKTD181IIDHSFAVEW MQDFEAFQDA LNQETTYVSN LTRSMSLVLD EFYSSLRVVG VSAVLGTGLD241ELFVQVTSAA EEYEREYRPE YERLKKSLAN AESQQQREQL ERLRKDMGSV ALDAGTAKDS301LSPVLHPSDL ILTRGTLDEE DEEADSDTDD IDHRVTEESH EEPAFQNFMQ ESMAQYWKRN361NKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
362 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.