GPCRW / GPR18 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G119180-100
Catalog Number: LS-G119180
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR18 (NM_001098200), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GPR18 | GPCRW | G protein-coupled receptor 18 | NAGly receptor | G-protein coupled receptor 18 | N-arachidonyl glycine receptor
- Origin Species
- Human
- NCBI GenBank
- NM_005292
- SwissProt
- Q14330
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MITLNNQDQP VPFNSSHPDE YKIAALVFYS CIFIIGLFVN ITALWVFSCT TKKRTTVTIY61MMNVALVDLI FIMTLPFRMF YYAKDEWPFG EYFCQILGAL TVFYPSIALW LLAFISADRY121MAIVQPKYAK ELKNTCKAVL ACVGVWIMTL TTTTPLLLLY KDPDKDSTPA TCLKISDIIY181LKAVNVLNLT RLTFFFLIPL FIMIGCYLVI IHNLLHGRTS KLKPKVKEKS IRIIITLLVQ241VLVCFMPFHI CFAFLMLGTG ENSYNPWGAF TTFLMNLSTC LDVILYYIVS KQFQARVISV301MLYRNYLRSM RRKSFRSGSL RSLSNINSEM LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
331 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.