Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPC6 / Glypican 6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G100625-100
Catalog Number: LS-G100625
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Glypican 6(GPC6) (NM_005708), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GPC6 | Glypican proteoglycan 6 | OMIMD1 | Glypican 6 | Glypican-6
- Origin Species
- Human
- NCBI GenBank
- NM_005708
- SwissProt
- Q9Y625
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.74 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPSWIGAVIL PLLGLLLSLP AGADVKARSC GEVRQAYGAK GFSLADIPYQ EIAGEHLRIC61PQEYTCCTTE MEDKLSQQSK LEFENLVEET SHFVRTTFVS RHKKFDEFFR ELLENAEKSL121NDMFVRTYGM LYMQNSEVFQ DLFTELKRYY TGGNVNLEEM LNDFWARLLE RMFQLINPQY181HFSEDYLECV SKYTDQLKPF GDVPRKLKIQ VTRAFIAART FVQGLTVGRE VANRVSKVSP241TPGCIRALMK MLYCPYCRGL PTVRPCNNYC LNVMKGCLAN QADLDTEWNL FIDAMLLVAE301RLEGPFNIES VMDPIDVKIS EAIMNMQENS MQVSAKVFQG CGQPKPAPAL RSARSAPENF361NTRFRPYNPE ERPTTAAGTS LDRLVTDIKE KLKLSKKVWS ALPYTICKDE SVTAGTSNEE421ECWNGHSKAR YLPEIMNDGL TNQINNPEVD VDITRPDTFI RQQIMALRVM TNKLKNAYNG481NDVNFQDTSD ESSGSGSGSG CMDDVCPTEF EFVTTEAPAV DPDRREVDSS AAQRGHSLLS541WSLTCIVLAL QRLCRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
555 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.