Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
GPC3 / Glypican 3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128268-100
Catalog Number: LS-G128268
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Glypican 3(GPC3) (NM_001164617), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DGSX | Glypican proteoglycan 3 | GPC3 | Glypican 3 | Glypican-3 | GTR2-2 | Intestinal protein OCI-5 | SGB | SGBS1 | SGBS | MXR7 | OCI-5 | OCI5 | SDYS | Secreted glypican-3 | Heparan sulphate proteoglycan
- Origin Species
- Human
- NCBI GenBank
- NM_004484
- SwissProt
- P51654
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.41 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGTVRTACL VVAMLLSLDF PGQAQPPPPP PDATCHQVRS FFQRLQPGLK WVPETPVPGS61DLQVCLPKGP TCCSRKMEEK YQLTARLNME QLLQSASMEL KFLIIQNAAV FQEAFEIVVR121HAKNYTNAMF KNNYPSLTPQ AFEFVGEFFT DVSLYILGSD INVDDMVNEL FDSLFPVIYT181QLMNPGLPDS ALDINECLRG ARRDLKVFGN FPKLIMTQVS KSLQVTRIFL QALNLGIEVI241NTTDHLKFSK DCGRMLTRMW YCSYCQGLMM VKPCGGYCNV VMQGCMAGVV EIDKYWREYI301LSLEELVNGM YRIYDMENVL LGLFSTIHDS IQYVQKNAGK LTTTETEKKI WHFKYPIFFL361CIGLDLQIGK LCAHSQQRQY RSAYYPEDLF IDKKVLKVAH VEHEETLSSR RRELIQKLKS421FISFYSALPG YICSHSPVAE NDTLCWNGQE LVERYSQKAA RNGMKNQFNL HELKMKGPEP481VVSQIIDKLK HINQLLRTMS MPKGRVLDKN LDEEGFESGD CGDDEDECIG GSGDGMIKVK541NQLRFLAELA YDLDVDDAPG NSQQATPKDN EISTFHNLGN VHSPLKLLTS MAISVVCFFF601LVHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
603 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.