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GPATCH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPATCH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GPATCH2 antibodies →
$725.00each
Catalog Number: LS-G112026-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPATCH2 (NM_018040), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 110 | CT110 | GPATCH2 | G patch domain containing 2 | GPATC2 | PPP1R30 | RP11-361K17.1
Origin Species
Human
NCBI GenBank
NM_018040
SwissProt
Q9NW75

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFGAAGRQPI GAPAAGNSWH FSRTMEELVH DLVSALEESS EQARGGFAET GDHSRSISCP
61LKRQARKRRG RKRRSYNVHH PWETGHCLSE GSDSSLEEPS KDYRENHNNN KKDHSDSDDQ
121MLVAKRRPSS NLNNNVRGKR PLWHESDFAV DNVGNRTLRR RRKVKRMAVD LPQDISNKRT
181MTQPPEGCRD QDMDSDRAYQ YQEFTKNKVK KRKLKIIRQG PKIQDEGVVL ESEETNQTNK
241DKMECEEQKV SDELMSESDS SSLSSTDAGL FTNDEGRQGD DEQSDWFYEK ESGGACGITG
301VVPWWEKEDP TELDKNVPDP VFESILTGSF PLMSHPSRRG FQARLSRLHG MSSKNIKKSG
361GTPTSMVPIP GPVGNKRMVH FSPDSHHHDH WFSPGARTEH DQHQLLRDNR AERGHKKNCS
421VRTASRQTSM HLGSLCTGDI KRRRKAAPLP GPTTAGFVGE NAQPILENNI GNRMLQNMGW
481TPGSGLGRDG KGISEPIQAM QRPKGLGLGF PLPKSTSATT TPNAGKSA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

528 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.