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GPAT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

GPAT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all GPAT2 antibodies →
$504.00each
Catalog Number: LS-G102605-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPAT 2(GPAT2) (NM_207328), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ALPAAB | Cancer/testis antigen 123 | BSCL | BSCL1 | CT123 | GPAT-2 | GPAT2 | XGPAT1
Origin Species
Human
NCBI GenBank
NM_207328
SwissProt
Q6NUI2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLEGRCQTQP RSSPSGREAS LWSSGFGMKL EAVTPFLGKY RPFVGRCCQT CTPKSWESLF
61HRSITDLGFC NVILVKEENT RFRGWLVRRL CYFLWSLEQH IPPCQDVPQK IMESTGVQNL
121LSGRVPGGTG EGQVPDLVKK EVQRILGHIQ APPRPFLVRL FSWALLRFLN CLFLNVQLHK
181GQMKMVQKAA QAGLPLVLLS THKTLLDGIL LPFMLLSQGL GVLRVAWDSR ACSPALRALL
241RKLGGLFLPP EASLSLDSSE GLLARAVVQA VIEQLLVSGQ PLLIFLEEPP GALGPRLSAL
301GQAWVGFVVQ AVQVGIVPDA LLVPVAVTYD LVQDAPCDID HASAPLGLWT GALAVLRSLW
361SRWGCSHRIC SRVHLAQPFS LQEYIVSARS CWGGRQTLEQ LLQPIVLGQC TAVPDTEKEQ
421EWTPITGPLL ALKEEDQLLV RRLSCHVLSA SVGSSAVMST AIMATLLLFK HQKLLGEFSW
481LTEEILLRGF DVGFSGQLRS LLQHSLSLLR AHVALLRIRQ GDLLVVPQPG PGLTHLAQLS
541AELLPVFLSE AVGACAVRGL LAGRVPPQGP WELQGILLLS QNELYRQILL LMHLLPQDLL
601LLKPCQSSYC YCQEVLDRLI QCGLLVAEET PGSRPACDTG RQRLSRKLLW KPSGDFTDSD
661SDDFGEADGR YFRLSQQSHC PDFFLFLCRL LSPLLKAFAQ AAAFLRQGQL PDTELGYTEQ
721LFQFLQATAQ EEGIFECADP KLAISAVWTF RDLGVLQQTP SPAGPRLHLS PTFASLDNQE
781KLEQFIRQFI CS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

792 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.